D
IPR006678

tRNA intron endonuclease, N-terminal

InterPro entry
Short nametRNA_intron_Endonuc_N
Overlapping
homologous
superfamilies
 

Description

This entry represents a 2-layer α/β domain found at the N terminus of the homotetrameric tRNA-intron endonucleases
[2]
, and as domains 1 (N-terminal) and 3 in the homodimeric enzymes
[3]
. tRNA-intron endonucleases (
3.1.27.9
) remove tRNA introns by cleaving pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-hydroxyl termini
[1]
. These enzymes recognise a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp
[4]
. Although homotetrameric enzymes contain four active sites, only two participate in the cleavage, and should therefore, be considered as a dimer of dimers.

References

1.Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems. Kleman-Leyer K, Armbruster DW, Daniels CJ. Cell 89, 839-47, (1997). View articlePMID: 9200602

2.Crystal structure and evolution of a transfer RNA splicing enzyme. Li H, Trotta CR, Abelson J. Science 280, 279-84, (1998). View articlePMID: 9535656

3.RNA recognition and cleavage by a splicing endonuclease. Xue S, Calvin K, Li H. Science 312, 906-10, (2006). View articlePMID: 16690865

4.Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2(1)2(1)2. Zhang Y, Li H. Acta Crystallogr. D Biol. Crystallogr. 60, 447-52, (2004). View articlePMID: 14993668

GO terms

Cross References

Contributing Member Database Entry
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.