F
IPR012838

Pyruvate formate-lyase 1 activating enzyme

InterPro entry
Short namePFL1_activating
Overlapping
homologous
superfamilies
 
family relationships

Description

Pyruvate formate lyase-activating enzyme
1.97.1.4
is a 28kDa monomeric protein which is involved in the anerobic glucose metabolism pathway. It is a member of the radical S-adenosylmethionine family of enzymes which initiate radical catalysis. It is necessary to activate pyruvate formate lyase (PFL), which catalyses the conversion of pyruvate and coenzyme A to formate and acetyl CoA.

Pyruvate formate lyase activating enzyme functions by catalysing the formation of the Gly734 radical of PFL. It utilises a [4Fe-4S] cluster and adenosylmethionine to generate a putative adenosyl radical, which extracts a hydrogen atom from Gly734 on PFL, thus activating PFL for catalysis
[1]
. Members of this family are iron-sulphur proteins with a radical-SAM domain.

References

1.Pyruvate formate-lyase activating enzyme: elucidation of a novel mechanism for glycyl radical formation. Buis JM, Broderick JB. Arch. Biochem. Biophys. 433, 288-96, (2005). View articlePMID: 15581584

GO terms

biological process

  • None

cellular component

  • None

Cross References

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