D
IPR013257

Set2 Rpb1 interacting domain

InterPro entry
Short nameSRI
Overlapping
homologous
superfamilies
 

Description

The SRI (Set2 Rpb1 interacting) domain mediates RNA polymerase II interaction and couples histone H3K36 methylation with transcript elongation
[1]
. This domain is conserved from yeast to humans. Members of this family form a compact, closed three-helix bundle, with an up-down-up topology. The first and second helices are antiparallel to each other and are of similar length; the third helix, which is packed across helices α1 and α2 is slightly shorter, consisting of only 15 amino acids. Most conserved hydrophobic residues are largely buried in the interior of the structure and form an extensive and contiguous hydrophobic core that stabilises the packing of the three-helix bundle. This domain mediates RNA polymerase II interaction and couples histone H3K36 methylation with transcript elongation
[2, 4]
.

This domain is also found in ATP-dependent DNA helicase Q5 (RECQ5), which enables REQ5 tointeract with Pol II
[3]
.

References

1.A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation. Kizer KO, Phatnani HP, Shibata Y, Hall H, Greenleaf AL, Strahl BD. Mol. Cell. Biol. 25, 3305-16, (2005). View articlePMID: 15798214

2.Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1. Li M, Phatnani HP, Guan Z, Sage H, Greenleaf AL, Zhou P. Proc. Natl. Acad. Sci. U.S.A. 102, 17636-41, (2005). View articlePMID: 16314571

3.Insights into the RecQ helicase mechanism revealed by the structure of the helicase domain of human RECQL5. Newman JA, Aitkenhead H, Savitsky P, Gileadi O. Nucleic Acids Res 45, 4231-4243, (2017). View articlePMID: 28100692

4.Structure and carboxyl-terminal domain (CTD) binding of the Set2 SRI domain that couples histone H3 Lys36 methylation to transcription. Vojnic E, Simon B, Strahl BD, Sattler M, Cramer P. J. Biol. Chem. 281, 13-5, (2006). View articlePMID: 16286474

GO terms

molecular function

  • None

cellular component

Cross References

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