D
IPR019576

Pyridoxine 5'-phosphate oxidase, dimerisation, C-terminal

InterPro entry
Short namePyridoxamine_oxidase_dimer_C
Overlapping
homologous
superfamilies
 

Description

Pyridoxamine 5'-phosphate oxidase (
1.4.3.5
) is an enzyme that is involved in the de novo synthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. It oxidizes pyridoxamine-5-P (PMP) and pyridoxine-5-P (PNP) to pyridoxal-5-P. The enzyme requires the presence of flavin mononucleotide (FMN) as a cofactor, although there is some evidence that coenzyme F420 may perform this role in some species
[2]
.

The sequences of the enzyme from bacterial (genes pdxH or fprA)
[3]
and fungal (gene PDX3)
[4]
sources show that this protein has been highly conserved throughout evolution. PdxH is evolutionary related
[5]
to one of the enzymes in the phenazine biosynthesis protein pathway, phzD (also known as phzG).

This entry represents one of the two dimerisation regions of the protein, located at the edge of the dimer interface, at the C terminus, being the last three β strands, S6, S7, and S8 along with the last three residues to the end. In
P21159
, S6 runs from residues 178-192, S7 from 200-206 and S8 from 211-215. the extended loop, of residues 167-177 may well be involved in the pocket formed between the two dimers that positions the FMN molecule
[6]
. To date, the only time functional oxidase or phenazine biosynthesis activities have been experimentally demonstrated is when the sequences contain both
PF01243
and
PF10590
. It is unknown the role performed by each domain in bringing about molecular functions of either oxidase or phenazine activity
[1]
.

References

1.Experimental Evidence for a Revision in the Annotation of Putative Pyridoxamine 5'-Phosphate Oxidases P(N/M)P from Fungi. Domitrovic T, Raymundo DP, da Silva TF, Palhano FL. PLoS ONE 10, e0136761, (2015). View articlePMID: 26327315

2.Unexpected abundance of coenzyme F(420)-dependent enzymes in Mycobacterium tuberculosis and other actinobacteria. Selengut JD, Haft DH. J. Bacteriol. 192, 5788-98, (2010). View articlePMID: 20675471

3.Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations. Lam HM, Winkler ME. J. Bacteriol. 174, 6033-45, (1992). View articlePMID: 1356963

4.Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase. Loubbardi A, Marcireau C, Karst F, Guilloton M. J. Bacteriol. 177, 1817-23, (1995). View articlePMID: 7896706

5.Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84. Pierson LS 3rd, Gaffney T, Lam S, Gong F. FEMS Microbiol. Lett. 134, 299-307, (1995). View articlePMID: 8586283

6.X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with FMN at 1.8 A resolution. Safo MK, Mathews I, Musayev FN, di Salvo ML, Thiel DJ, Abraham DJ, Schirch V. Structure 8, 751-62, (2000). View articlePMID: 10903950

Cross References

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