S
IPR020084

NUDIX hydrolase, conserved site

InterPro entry
Short nameNUDIX_hydrolase_CS

Description

MutT is a small bacterial protein (~12-15Kd) involved in the GO system
[1]
responsible for removing an oxidatively damaged form of guanine (8-hydroxy- guanine or 7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with near equal efficiency, leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate, with the concomitant release of pyrophosphate. A short conserved N-terminal region of mutT (designated the MutT domain) is also found in a variety of other prokaryotic, viral and eukaryotic proteins
[2, 3, 4, 5]
.

The generic name `NUDIX hydrolases' (NUcleoside DIphosphate linked to some other moiety X) has been coined for this domain family
[6]
. The family can be divided into a number of subgroups, of which MutT anti- mutagenic activity represents only one type; most of the rest hydrolyse diverse nucleoside diphosphate derivatives (including ADP-ribose, GDP- mannose, TDP-glucose, NADH, UDP-sugars, dNTP and NTP).

This signature covers the core region of the NUDIX domain and contains four conserved glutamate residues
[2]
. The region spanned by this signature could be part of the active centre of a family of pyrophosphate-releasing NTPases.

References

1.The GO system protects organisms from the mutagenic effect of the spontaneous lesion 8-hydroxyguanine (7,8-dihydro-8-oxoguanine). Michaels ML, Miller JH. J. Bacteriol. 174, 6321-5, (1992). View articlePMID: 1328155

2.A highly conserved sequence motif defining the family of MutT-related proteins from eubacteria, eukaryotes and viruses. Koonin EV. Nucleic Acids Res. 21, 4847, (1993). View articlePMID: 8233837

3.Characterization of the mutX gene of Streptococcus pneumoniae as a homologue of Escherichia coli mutT, and tentative definition of a catalytic domain of the dGTP pyrophosphohydrolases. Mejean V, Salles C, Bullions LC, Bessman MJ, Claverys JP. Mol. Microbiol. 11, 323-30, (1994). View articlePMID: 8170394

4.Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. Sakumi K, Furuichi M, Tsuzuki T, Kakuma T, Kawabata S, Maki H, Sekiguchi M. J. Biol. Chem. 268, 23524-30, (1993). View articlePMID: 8226881

5.The MutT motif family of nucleotide phosphohydrolases in man and human pathogens (review). McLennan AG. Int. J. Mol. Med. 4, 79-89, (1999). PMID: 10373642

6.The MutT proteins or "Nudix" hydrolases, a family of versatile, widely distributed, "housecleaning" enzymes. Bessman MJ, Frick DN, O'Handley SF. J. Biol. Chem. 271, 25059-62, (1996). View articlePMID: 8810257

GO terms

biological process

  • None

cellular component

  • None

Cross References

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