D
IPR025162

Recombination activating protein 2, PHD domain

InterPro entry
Short nameRAG2_PHD
Overlapping
homologous
superfamilies
 

Description

RAG-2 is an essential component of the lymphoid-specific recombination activating gene RAG1/2 V(D)J recombinase mediating antigen-receptor gene assembly. It contains an acidic hinge region implicated in histone-binding, a non-canonical plant homeodomain (PHD) finger followed by a C-terminal extension of 40 amino acids that is essential for phosphoinositide (PtdIns)-binding.
[1, 2, 3]
.

This domain, a non-canonical plant homeodomain (PHD) finger, is found at the C terminus of the Recombination activating gene 2 (RAG2) protein. The PHD finger is a chromatin-binding module that has been shown bound to histone H3 trimethylated at lysine 4 (H3K4me3) and influences V(D)J recombination
[1]
.

References

1.RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Matthews AG, Kuo AJ, Ramon-Maiques S, Han S, Champagne KS, Ivanov D, Gallardo M, Carney D, Cheung P, Ciccone DN, Walter KL, Utz PJ, Shi Y, Kutateladze TG, Yang W, Gozani O, Oettinger MA. Nature 450, 1106-10, (2007). View articlePMID: 18033247

2.A PHD finger motif in the C terminus of RAG2 modulates recombination activity. Elkin SK, Ivanov D, Ewalt M, Ferguson CG, Hyberts SG, Sun ZY, Prestwich GD, Yuan J, Wagner G, Oettinger MA, Gozani OP. J. Biol. Chem. 280, 28701-10, (2005). View articlePMID: 15964836

3.The plant homeodomain finger of RAG2 recognizes histone H3 methylated at both lysine-4 and arginine-2. Ramon-Maiques S, Kuo AJ, Carney D, Matthews AG, Oettinger MA, Gozani O, Yang W. Proc. Natl. Acad. Sci. U.S.A. 104, 18993-8, (2007). View articlePMID: 18025461

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