S
IPR033126

Glycosyl hydrolases family 9, Asp/Glu active sites

InterPro entry
Short nameGlyco_hydro_9_Asp/Glu_AS

Description

The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases, cellobiohydrolases (exoglucanases), or xylanases
[6, 1]
. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family E
[2]
or as the glycosyl hydrolases family 9
[3]
.

Two of the most conserved regions in these enzymes are centred on conserved residues which have been shown
[4, 5]
, in the endoglucanase D from Cellulomonas thermocellum, to be important for the catalytic activity. The first region contains an active site histidine and the second region contains two catalytically important residues: an aspartate and a glutamate. This entry represents the second region.

References

1.Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RA. Microbiol. Rev. 55, 303-15, (1991). View articlePMID: 1886523

2.Cellulase families revealed by hydrophobic cluster analysis. Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon JP. Gene 81, 83-95, (1989). View articlePMID: 2806912

3.A classification of glycosyl hydrolases based on amino acid sequence similarities. Henrissat B. Biochem. J. 280 ( Pt 2), 309-16, (1991). View articlePMID: 1747104

4.Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum. Tomme P, Chauvaux S, Beguin P, Millet J, Aubert JP, Claeyssens M. J. Biol. Chem. 266, 10313-8, (1991). View articlePMID: 2037583

5.Modification of catalytically important carboxy residues in endoglucanase D from Clostridium thermocellum. Tomme P, van Beeumen J, Claeyssens M. Biochem. J. 285 ( Pt 1), 319-24, (1992). View articlePMID: 1637316

6.Molecular biology of cellulose degradation. Beguin P. Annu. Rev. Microbiol. 44, 219-48, (1990). View articlePMID: 2252383

Cross References

Contributing Member Database Entry
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.