F
IPR033697

Ribonuclease T2, eukaryotic

InterPro entry
Short nameRibonuclease_T2_eukaryotic
Overlapping
homologous
superfamilies
 
family relationships

Description

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases)
[1, 2, 3, 4, 5]
. Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sissile bond, respectively.

This entry includes the eukaryotic RNase T2 family members.

References

1.Ribonucleases from T2 family. Deshpande RA, Shankar V. Crit. Rev. Microbiol. 28, 79-122, (2002). PMID: 12109772

2.Ribonuclease T2. Irie M, Ohgi K. Meth. Enzymol. 341, 42-55, (2001). View articlePMID: 11582795

3.Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 A resolution. Nakagawa A, Tanaka I, Sakai R, Nakashima T, Funatsu G, Kimura M. Biochim. Biophys. Acta 1433, 253-60, (1999). View articlePMID: 10446375

4.Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity. Numata T, Suzuki A, Kakuta Y, Kimura K, Yao M, Tanaka I, Yoshida Y, Ueda T, Kimura M. Biochemistry 42, 5270-8, (2003). View articlePMID: 12731868

5.Characterization of Rny1, the Saccharomyces cerevisiae member of the T2 RNase family of RNases: unexpected functions for ancient enzymes? MacIntosh GC, Bariola PA, Newbigin E, Green PJ. Proc. Natl. Acad. Sci. U.S.A. 98, 1018-23, (2001). View articlePMID: 11158587

Cross References

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