D
IPR043608

Nonstructural protein 15, middle domain, coronavirus

InterPro entry
Short nameCoV_NSP15_M
Overlapping
homologous
superfamilies
 
domain relationships

Description

The unique coronavirus transcription/replication machinery comprised of multiple virus-encoded non structural proteins (NSP) plays a vital role during initial and intermediate phases of the viral life cycle. NSP15 forms a hexamer made of dimers of trimers which is suggested to be a functional unit, responsible for the endoribonuclease activity. The NSP15 monomer consists of three domains: N-terminal, middle and C-terminal
[5, 2]
. The catalytic function of NSP15 resides in the C-terminal NendoU domain. The active site carries six key residues conserved among SARS-CoV-2, SARS-CoV and MERS-CoV, suggesting that its activity is important for sustained replication in the host
[4, 3]
.

This entry represents the non-catalytic middle domain from NSP15. This domain is formed by ten β-strands organised into threeβ-hairpins. It creates concave surfaces that may serve as interaction hubs with other proteins and RNA
[4, 1]
.

References

1.Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Kim Y, Jedrzejczak R, Maltseva NI, Wilamowski M, Endres M, Godzik A, Michalska K, Joachimiak A. Protein Sci. (2020). PMID: 32304108

2.Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics. Pillon MC, Frazier MN, Dillard LB, Williams JG, Kocaman S, Krahn JM, Perera L, Hayne CK, Gordon J, Stewart ZD, Sobhany M, Deterding LJ, Hsu AL, Dandey VP, Borgnia MJ, Stanley RE. Nat Commun 12, 636, (2021). PMID: 33504779

3.Biochemical characterization of arterivirus nonstructural protein 11 reveals the nidovirus-wide conservation of a replicative endoribonuclease. Nedialkova DD, Ulferts R, van den Born E, Lauber C, Gorbalenya AE, Ziebuhr J, Snijder EJ. J. Virol. 83, 5671-82, (2009). PMID: 19297500

4.An "Old" protein with a new story: Coronavirus endoribonuclease is important for evading host antiviral defenses. Deng X, Baker SC. Virology 517, 157-163, (2018). PMID: 29307596

5.New antiviral target revealed by the hexameric structure of mouse hepatitis virus nonstructural protein nsp15. Xu X, Zhai Y, Sun F, Lou Z, Su D, Xu Y, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z. J. Virol. 80, 7909-17, (2006). View articlePMID: 16873248

Cross References

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