Member database | CATH-Gene3D |
CATH-Gene3D type | homologous superfamily |
Description Imported from IPR009017
The green fluorescent-like protein family consists of fluorescent proteins and non-fluorescent chromoproteins, derived from several species of Cnidarians, as well as certain diazotrophic bacteria
[2, 1]. These proteins range in their absorption wavelength maximum, and are often classified by their colour: green, yellow, red and purple-blue. These colour differences arise from changes in the structure of the chromophore, which is generated internally by auto-catalysis. The chromophore comprises Ser65-Tyr66-Gly67 in Aequorea victoria (Jellyfish), which forms a five-member ring after its modification. In the bioluminescent organism A. victoria, GFP acts to transform the blue light emitted from aequorin into green light. However, most organisms with GFP-like molecules are not bioluminescent, and in some cases are not even fluorescent. These proteins all display a β-can structure, which surrounds the chromophore and acts to shield it against quenching agents.
References Imported from IPR009017
1.Green fluorescent protein-like proteins in reef Anthozoa animals. Miyawaki A. Cell Struct. Funct. 27, 343-7, (2002). View articlePMID: 12502888
2.Diversity and evolution of the green fluorescent protein family. Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, Lukyanov SA, Matz MV. Proc. Natl. Acad. Sci. U.S.A. 99, 4256-61, (2002). View articlePMID: 11929996
Further reading
3. Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1. Hopf M, Gohring W, Ries A, Timpl R, Hohenester E. Nat. Struct. Biol. 8, 634-40, (2001). View articlePMID: 11427896
Integrated to
External Links
Representative structure
6l27: X-ray crystal structure of the mutant green fluorescent protein