G3DSA:3.10.28.10

Homing endonucleases

CATH-Gene3D entry
Member databaseCATH-Gene3D
CATH-Gene3D typehomologous superfamily

Description
Imported from IPR027434

Homing endonucleases are rare-cutting enzymes encoded by inteins and introns. They are found inserted within host genes in eukaryotes, bacteria, archaebacteria and viruses
[4]
. By making a site-specific double-strand break in the intronless or inteinless alleles, these nucleases create recombinogenic ends which engage in a gene conversion process that duplicates the intron or intein
[1, 2]
. There are four families of homing endonucleases classified by the conserved sequence motifs LAGLIDADG, GIY-YIG, H-N-H and His-Cys box
[1, 5]
. Endonucleases of the DOD family contain one or two copies of the 10-residue sequence known as a dodecapeptide or LAGLIDADG motif. LAGLIDADG endonucleases are either monomers, such as I-SceI, that are composed of two pseudo symmetric subdomains, or homodimers, such as I-CreI. In both cases, the LAGLIDADG endonuclease folds into a β-saddle architecture, a common motif for nucleic acid binding proteins
[3]
.

This superfamily represents the homing endonuclease domain.

References
Imported from IPR027434

1.Homing endonucleases: keeping the house in order. Belfort M, Roberts RJ. Nucleic Acids Res. 25, 3379-88, (1997). View articlePMID: 9254693

2.Homing endonucleases: structure, function and evolution. Jurica MS, Stoddard BL. Cell. Mol. Life Sci. 55, 1304-26, (1999). View articlePMID: 10487208

3.Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway. Moure CM, Gimble FS, Quiocho FA. Nucleic Acids Res. 36, 3287-96, (2008). View articlePMID: 18424798

4.Invasion of a multitude of genetic niches by mobile endonuclease genes. Gimble FS. FEMS Microbiol. Lett. 185, 99-107, (2000). View articlePMID: 10754232

5.The conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis. Surdova K, Gamba P, Claessen D, Siersma T, Jonker MJ, Errington J, Hamoen LW. J. Bacteriol. 195, 5450-60, (2013). View articlePMID: 24097947

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.