G3DSA:3.20.20.100

NADP-dependent oxidoreductase domain

CATH-Gene3D entry
Member databaseCATH-Gene3D
CATH-Gene3D typehomologous superfamily

Description
Imported from IPR036812

The aldo-keto reductase family includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others
[3]
. All possess a similar structure, with a β-α-β fold characteristic of nucleotide binding proteins
[2]
. The fold comprises a parallel β8/α8 barrel, which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the β-sheet, the substrate being bound in an extended conformation. The hydrophobic nature of the pocket favours aromatic and apolar substrates over highly polar ones
[1]
.

Binding of the NADPH coenzyme causes a massive conformational change, reorienting a loop, effectively locking the coenzyme in place. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases
[5]
.

Some proteins of this entry contain a K+ ion channel beta chain regulatory domain; these are reported to have oxidoreductase activity
[4]
.

References
Imported from IPR036812

1.An unlikely sugar substrate site in the 1.65 A structure of the human aldose reductase holoenzyme implicated in diabetic complications. Wilson DK, Bohren KM, Gabbay KH, Quiocho FA. Science 257, 81-4, (1992). View articlePMID: 1621098

2.Sequence analysis of bovine lens aldose reductase. Schade SZ, Early SL, Williams TR, Kezdy FJ, Heinrikson RL, Grimshaw CE, Doughty CC. J. Biol. Chem. 265, 3628-35, (1990). View articlePMID: 2105951

3.The aldo-keto reductase superfamily. cDNAs and deduced amino acid sequences of human aldehyde and aldose reductases. Bohren KM, Bullock B, Wermuth B, Gabbay KH. J. Biol. Chem. 264, 9547-51, (1989). View articlePMID: 2498333

4.Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels. Gulbis JM, Zhou M, Mann S, MacKinnon R. Science 289, 123-7, (2000). View articlePMID: 10884227

5.The crystal structure of the aldose reductase.NADPH binary complex. Borhani DW, Harter TM, Petrash JM. J. Biol. Chem. 267, 24841-7, (1992). View articlePMID: 1447221

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