cd00383

DNA-binding effector domain of two-component system response regulators

CDD entry
Member databaseCDD
CDD typedomain
Short nametrans_reg_C
SetHTH

Description

Bacteria and some eukaryotes use two-component signal transduction systems to detect and respond to changes in the environment. The systems consists of a sensor histidine kinase and a response regulator. The former autophosphorylates a histidine residue on detecting an external stimulus. The phosphate is then transferred to an invariant aspartate residue in a highly conserved receiver domain of the response regulator. Phosphorylation activates a variable effector domain of the response regulator, which triggers the cellular response. This C-terminal effector domain belongs to the winged helix-turn-helix family of transcriptional regulators and contains DNA and RNA polymerase binding sites. Several dimers or monomers bind head to tail to small tandem repeats upstream of the genes. The RNA polymerase binding sites interact with the alpha or sigma subunit of RNA polymerase.
[2, 3, 4, 6, 5, 1]

References

1.The OmpR-family of proteins: insight into the tertiary structure and functions of two-component regulator proteins. Itou H, Tanaka I. J Biochem 129, 343-50, (2001). PMID: 11226872

2.Structure/function relationships in OmpR and other winged-helix transcription factors. Kenney LJ. Curr. Opin. Microbiol. 5, 135-41, (2002). View articlePMID: 11934608

3.Structural relationships in the OmpR family of winged-helix transcription factors. Martinez-Hackert E, Stock AM. J. Mol. Biol. 269, 301-12, (1997). View articlePMID: 9199401

4.The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor. Martinez-Hackert E, Stock AM. Structure 5, 109-24, (1997). View articlePMID: 9016718

5.A single-amino-acid substitution in the C terminus of PhoP determines DNA-binding specificity of the virulence-associated response regulator from Mycobacterium tuberculosis. Das AK, Pathak A, Sinha A, Datt M, Singh B, Karthikeyan S, Sarkar D. J Mol Biol 398, 647-56, (2010). PMID: 20363229

6.Structure of the DNA-binding domain of the response regulator PhoP from Mycobacterium tuberculosis. Wang S, Engohang-Ndong J, Smith I. Biochemistry 46, 14751-61, (2007). View articlePMID: 18052041

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