cd02619

Peptidase_C1

CDD entry
Member databaseCDD
CDD typedomain
Short namePeptidase_C1
SetPeptidase_C1

Description

C1 Peptidase family (MEROPS database nomenclature), also referred to as the papain family; composed of two subfamilies of cysteine peptidases (CPs), C1A (papain) and C1B (bleomycin hydrolase). Papain-like enzymes are mostly endopeptidases with some exceptions like cathepsins B, C, H and X, which are exopeptidases. Papain-like CPs have different functions in various organisms. Plant CPs are used to mobilize storage proteins in seeds while mammalian CPs are primarily lysosomal enzymes responsible for protein degradation in the lysosome. Papain-like CPs are synthesized as inactive proenzymes with N-terminal propeptide regions, which are removed upon activation. Bleomycin hydrolase (BH) is a CP that detoxifies bleomycin by hydrolysis of an amide group. It acts as a carboxypeptidase on its C-terminus to convert itself into an aminopeptidase and peptide ligase. BH is found in all tissues in mammals as well as in many other eukaryotes. It forms a hexameric ring barrel structure with the active sites imbedded in the central channel. Some members of the C1 family are proteins classified as non-peptidase homologs which lack peptidase activity or have missing active site residues.
[7, 1, 2, 8, 6, 5, 4, 3]

References

1.Alignment/phylogeny of the papain superfamily of cysteine proteases. Berti PJ, Storer AC. J Mol Biol 246, 273-83, (1995). PMID: 7869379

2.Family C1 cysteine proteases: biological diversity or redundancy? Nagler DK, Menard R. Biol Chem 384, 837-43, (2003). PMID: 12887050

3.MEROPS: the peptidase database. Rawlings ND, Tolle DP, Barrett AJ. Nucleic Acids Res 32, D160-4, (2004). PMID: 14681384

4.Gene cloning and characterization of PepC, a cysteine aminopeptidase from Streptococcus thermophilus, with sequence similarity to the eucaryotic bleomycin hydrolase. Chapot-Chartier MP, Rul F, Nardi M, Gripon JC. Eur. J. Biochem. 224, 497-506, (1994). View articlePMID: 7925365

5.The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase, aminopeptidase, and peptide ligase. Zheng W, Johnston SA, Joshua-Tor L. Cell 93, 103-9, (1998). View articlePMID: 9546396

6.Structural studies of cysteine proteases and their inhibitors. Grzonka Z, Jankowska E, Kasprzykowski F, Kasprzykowska R, Lankiewicz L, Wiczk W, Wieczerzak E, Ciarkowski J, Drabik P, Janowski R, Kozak M, Jaskolski M, Grubb A. Acta Biochim. Pol. 48, 1-20, (2001). PMID: 11440158

7.Evolutionary lines of cysteine peptidases. Barrett AJ, Rawlings ND. Biol. Chem. 382, 727-33, (2001). View articlePMID: 11517925

8.Structure-function relationships in class CA1 cysteine peptidase propeptides. Wiederanders B. Acta Biochim. Pol. 50, 691-713, (2003). View articlePMID: 14515150

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