cd02869

Pseudouridine synthase, RluA family

CDD entry
Member databaseCDD
CDD typedomain
Short namePseudoU_synth_RluA_like
SetPseudoU_synth

Description

This group is comprised of eukaryotic, bacterial and archeal proteins similar to eight site specific Escherichia coli pseudouridine synthases: RsuA, RluA, RluB, RluC, RluD, RluE, RluF and TruA. Pseudouridine synthases catalyze the isomerization of specific uridines in a n RNA molecule to pseudouridines (5-ribosyluracil, psi) requiring no cofactors. E. coli RluC for example makes psi955, 2504 and 2580 in 23S RNA. Some psi sites such as psi1917 in 23S RNA made by RluD are universally conserved. Other psi sites occur in a more restricted fashion, for example psi2819 in 21S mitochondrial ribosomal RNA made by S. cerevisiae Pus5p is only found in mitochondrial large subunit rRNAs from some other species and in gram negative bacteria. The E. coli counterpart of this psi residue is psi2580 in 23S rRNA. psi2604in 23S RNA made by RluF has only been detected in E.coli.
[7, 5, 3, 2, 8, 10, 6, 9, 1, 4]

References

1.Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli. Mizutani K, Machida Y, Unzai S, Park SY, Tame JR. Biochemistry 43, 4454-63, (2004). View articlePMID: 15078091

2.The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA. Conrad J, Sun D, Englund N, Ofengand J. J. Biol. Chem. 273, 18562-6, (1998). View articlePMID: 9660827

3.A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe). Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J. RNA 1, 437-48, (1995). View articlePMID: 7493321

4.Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP. Sivaraman J, Sauve V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A. Nat. Struct. Biol. 9, 353-8, (2002). PMID: 11953756

5.16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases. Conrad J, Niu L, Rudd K, Lane BG, Ofengand J. RNA 5, 751-63, (1999). PMID: 10376875

6.Cloning and characterization of the 23S RNA pseudouridine 2633 synthase from Bacillus subtilis. Niu L, Lane BG, Ofengand J. Biochemistry 38, 629-35, (1999). PMID: 9888802

7.Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli. Del Campo M, Kaya Y, Ofengand J. RNA 7, 1603-15, (2001). PMID: 11720289

8.Isolation and properties of Escherichia coli 23S-RNA pseudouridine 1911, 1915, 1917 synthase (RluD). Wrzesinski J, Bakin A, Ofengand J, Lane BG. IUBMB Life 50, 33-7, (2000). PMID: 11087118

9.Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of psi(2819) in 21S mitochondrial ribosomal RNA. Ansmant I, Massenet S, Grosjean H, Motorin Y, Branlant C. Nucleic Acids Res 28, 1941-6, (2000). PMID: 10756195

10.A second function for pseudouridine synthases: A point mutant of RluD unable to form pseudouridines 1911, 1915, and 1917 in Escherichia coli 23S ribosomal RNA restores normal growth to an RluD-minus strain. Gutgsell NS, Del Campo M, Raychaudhuri S, Ofengand J. RNA 7, 990-8, (2001). PMID: 11453071

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