cd08378

C2 domain second repeat found in Multiple C2 domain and Transmembrane region Proteins (MCTP); plant subset

CDD entry
Member databaseCDD
CDD typedomain
Short nameC2B_MCTP_PRT_plant
SetC2

Description

MCTPs are involved in Ca2+ signaling at the membrane. Plant-MCTPs are composed of a variable N-terminal sequence, four C2 domains, two transmembrane regions (TMRs), and a short C-terminal sequence. It is one of four protein classes that are anchored to membranes via a transmembrane region; the others being synaptotagmins, extended synaptotagmins, and ferlins. MCTPs are the only membrane-bound C2 domain proteins that contain two functional TMRs. MCTPs are unique in that they bind Ca2+ but not phospholipids. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-II topology.
[8, 5, 1, 4, 7, 6, 2, 3]

References

1.Extending the C2 domain family: C2s in PKCs delta, epsilon, eta, theta, phospholipases, GAPs, and perforin. Ponting CP, Parker PJ. Protein Sci. 5, 162-6, (1996). View articlePMID: 8771209

2.Synaptotagmin: a calcium sensor on the synaptic vesicle surface. Brose N, Petrenko AG, Sudhof TC, Jahn R. Science 256, 1021-5, (1992). View articlePMID: 1589771

3.Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C. Perin MS, Fried VA, Mignery GA, Jahn R, Sudhof TC. Nature 345, 260-3, (1990). View articlePMID: 2333096

4.Bipartite Ca2+-binding motif in C2 domains of synaptotagmin and protein kinase C. Shao X, Davletov BA, Sutton RB, Sudhof TC, Rizo J. Science 273, 248-51, (1996). View articlePMID: 8662510

5.The C2 domain calcium-binding motif: structural and functional diversity. Nalefski EA, Falke JJ. Protein Sci. 5, 2375-90, (1996). View articlePMID: 8976547

6.Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold. Sutton RB, Davletov BA, Berghuis AM, Sudhof TC, Sprang SR. Cell 80, 929-38, (1995). View articlePMID: 7697723

7.Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins. Li C, Ullrich B, Zhang JZ, Anderson RG, Brose N, Sudhof TC. Nature 375, 594-9, (1995). View articlePMID: 7791877

8.C2-domains, structure and function of a universal Ca2+-binding domain. Rizo J, Sudhof TC. J. Biol. Chem. 273, 15879-82, (1998). View articlePMID: 9632630

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