cd15503

PHD finger 2 found in polycomb repressive complex 2 (PRC2)-associated polycomb-like (PCL) family proteins MTF2, PHF19, and similar proteins

CDD entry
Member databaseCDD
CDD typedomain
Short namePHD2_MTF2_PHF19_like
SetPHD_SF

Description

The PCL family includes PHD finger protein1 (PHF1) and its homologs metal-response element-binding transcription factor 2 (MTF2/PCL2) and PHF19/PCL3, which are accessory components of the Polycomb repressive complex 2 (PRC2) core complex and all contain an N-terminal Tudor domain followed by two plant homeodomain (PHD) fingers, and a C-terminal MTF2 domain. PCL proteins specifically recognize tri-methylated H3K36 (H3K36me3) through their N-terminal Tudor domains. The interaction between their Tudor domains and H3K36me3 is critical for both the targeting and spreading of PRC2 into active chromatin regions and for the maintenance of optimal repression of poised developmental genes where PCL proteins, H3K36me3, and H3K27me3 coexist. Moreover, unlike other PHD finger-containing proteins, the first PHD finger of PCL proteins do not display histone H3K4 binding affinity and they do not affect the Tudor domain binding to histones. This model corresponds to the second PHD finger.
[6, 1, 14, 9, 19, 15, 20, 16, 2, 7, 11, 13, 8, 21, 12, 17, 5, 18, 4, 3, 10]

References

1.H3K36me3 key to Polycomb-mediated gene silencing in lineage specification. Abed JA, Jones RS. Nat. Struct. Mol. Biol. 19, 1214-5, (2012). View articlePMID: 23211767

2.Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal. Hunkapiller J, Shen Y, Diaz A, Cagney G, McCleary D, Ramalho-Santos M, Krogan N, Ren B, Song JS, Reiter JF. PLoS Genet. 8, e1002576, (2012). View articlePMID: 22438827

3.The identification and localization of a human gene with sequence similarity to Polycomblike of Drosophila melanogaster. Coulson M, Robert S, Eyre HJ, Saint R. Genomics 48, 381-3, (1998). PMID: 9545646

4.Consistent rearrangement of chromosomal band 6p21 with generation of fusion genes JAZF1/PHF1 and EPC1/PHF1 in endometrial stromal sarcoma. Micci F, Panagopoulos I, Bjerkehagen B, Heim S. Cancer Res 66, 107-12, (2006). PMID: 16397222

5.Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo. Sarma K, Margueron R, Ivanov A, Pirrotta V, Reinberg D. Mol Cell Biol 28, 2718-31, (2008). PMID: 18285464

6.Tudor domains of the PRC2 components PHF1 and PHF19 selectively bind to histone H3K36me3. Qin S, Guo Y, Xu C, Bian C, Fu M, Gong S, Min J. Biochem. Biophys. Res. Commun. 430, 547-53, (2013). View articlePMID: 23228662

7.Mammalian polycomb-like Pcl2/Mtf2 is a novel regulatory component of PRC2 that can differentially modulate polycomb activity both at the Hox gene cluster and at Cdkn2a genes. Li X, Isono K, Yamada D, Endo TA, Endoh M, Shinga J, Mizutani-Koseki Y, Otte AP, Casanova M, Kitamura H, Kamijo T, Sharif J, Ohara O, Toyada T, Bernstein BE, Brockdorff N, Koseki H. Mol. Cell. Biol. 31, 351-64, (2011). View articlePMID: 21059868

8.The Tudor domain of the PHD finger protein 1 is a dual reader of lysine trimethylation at lysine 36 of histone H3 and lysine 27 of histone variant H3t. Kycia I, Kudithipudi S, Tamas R, Kungulovski G, Dhayalan A, Jeltsch A. J. Mol. Biol. 426, 1651-60, (2014). View articlePMID: 23954330

9.Functional characterization of human Polycomb-like 3 isoforms identifies them as components of distinct EZH2 protein complexes. Boulay G, Rosnoblet C, Guerardel C, Angrand PO, Leprince D. Biochem. J. 434, 333-42, (2011). View articlePMID: 21143197

10.Novel fusion of MYST/Esa1-associated factor 6 and PHF1 in endometrial stromal sarcoma. Panagopoulos I, Micci F, Thorsen J, Gorunova L, Eibak AM, Bjerkehagen B, Davidson B, Heim S. PLoS One 7, e39354, (2012). PMID: 22761769

11.Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation. Walker E, Chang WY, Hunkapiller J, Cagney G, Garcha K, Torchia J, Krogan NJ, Reiter JF, Stanford WL. Cell Stem Cell 6, 153-66, (2010). View articlePMID: 20144788

12.Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. Vaz Meirelles G, Ferreira Lanza DC, da Silva JC, Santana Bernachi J, Paes Leme AF, Kobarg J. J Proteome Res 9, 6298-316, (2010). PMID: 20873783

13.Molecular basis for H3K36me3 recognition by the Tudor domain of PHF1. Musselman CA, Avvakumov N, Watanabe R, Abraham CG, Lalonde ME, Hong Z, Allen C, Roy S, Nunez JK, Nickoloff J, Kulesza CA, Yasui A, Cote J, Kutateladze TG. Nat. Struct. Mol. Biol. 19, 1266-72, (2012). View articlePMID: 23142980

14.A novel human homologue of Drosophila polycomblike gene is up-regulated in multiple cancers. Wang S, Robertson GP, Zhu J. Gene 343, 69-78, (2004). View articlePMID: 15563832

15.Polycomb PHF19 binds H3K36me3 and recruits PRC2 and demethylase NO66 to embryonic stem cell genes during differentiation. Brien GL, Gambero G, O'Connell DJ, Jerman E, Turner SA, Egan CM, Dunne EJ, Jurgens MC, Wynne K, Piao L, Lohan AJ, Ferguson N, Shi X, Sinha KM, Loftus BJ, Cagney G, Bracken AP. Nat. Struct. Mol. Biol. 19, 1273-81, (2012). View articlePMID: 23160351

16.Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity. Ballare C, Lange M, Lapinaite A, Martin GM, Morey L, Pascual G, Liefke R, Simon B, Shi Y, Gozani O, Carlomagno T, Benitah SA, Di Croce L. Nat. Struct. Mol. Biol. 19, 1257-65, (2012). View articlePMID: 23104054

17.A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell. Hong Z, Jiang J, Lan L, Nakajima S, Kanno S, Koseki H, Yasui A. Nucleic Acids Res. 36, 2939-47, (2008). View articlePMID: 18385154

18.Role of hPHF1 in H3K27 methylation and Hox gene silencing. Cao R, Wang H, He J, Erdjument-Bromage H, Tempst P, Zhang Y. Mol. Cell. Biol. 28, 1862-72, (2008). View articlePMID: 18086877

19.PHF19 and Akt control the switch between proliferative and invasive states in melanoma. Ghislin S, Deshayes F, Middendorp S, Boggetto N, Alcaide-Loridan C. Cell Cycle 11, 1634-45, (2012). View articlePMID: 22487681

20.An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Cai L, Rothbart SB, Lu R, Xu B, Chen WY, Tripathy A, Rockowitz S, Zheng D, Patel DJ, Allis CD, Strahl BD, Song J, Wang GG. Mol. Cell 49, 571-82, (2013). View articlePMID: 23273982

21.Polycomb group protein PHF1 regulates p53-dependent cell growth arrest and apoptosis. Yang Y, Wang C, Zhang P, Gao K, Wang D, Yu H, Zhang T, Jiang S, Hexige S, Hong Z, Yasui A, Liu JO, Huang H, Yu L. J. Biol. Chem. 288, 529-39, (2013). View articlePMID: 23150668

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