cd17596

phosphoacceptor receiver (REC) domain of hydrogen uptake protein regulator (HupR)

CDD entry
Member databaseCDD
CDD typedomain
Short nameREC_HupR
SetREC

Description

Members of this subfamily are response regulator components of two-component systems that regulates hydrogenase activity, including HupR and HoxA. HupR is part of the HupT/HupR system that controls the synthesis of the membrane-bound [NiFe]hydrogenase, HupSL, of the photosynthetic bacterium Rhodobacter capsulatus. It belongs to the nitrogen regulatory protein C (NtrC) family of response regulators, which activate transcription by RNA polymerase (RNAP) in response to a change in the environment. HupR is an unusual member of this family as it activates transcription when unphosphorylated, and transcription is inhibited by phosphorylation. Proteins in this subfamily contain an N-terminal REC domain, a central sigma-54 interaction domain that lacks ATPase activity, and a C-terminal DNA-binding domain. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.
[6, 8, 3, 4, 7, 1, 5, 2]

References

1.Structural classification of bacterial response regulators: diversity of output domains and domain combinations. Galperin MY. J. Bacteriol. 188, 4169-82, (2006). View articlePMID: 16740923

2.Novel domains of the prokaryotic two-component signal transduction systems. Galperin MY, Nikolskaya AN, Koonin EV. FEMS Microbiol. Lett. 203, 11-21, (2001). View articlePMID: 11557134

3.The synthesis of Rhodobacter capsulatus HupSL hydrogenase is regulated by the two-component HupT/HupR system. Dischert W, Vignais PM, Colbeau A. Mol Microbiol 34, 995-1006, (1999). PMID: 10594824

4.A hydrogen-sensing system in transcriptional regulation of hydrogenase gene expression in Alcaligenes species. Lenz O, Strack A, Tran-Betcke A, Friedrich B. J Bacteriol 179, 1655-63, (1997). PMID: 9045826

5.Diversity of structure and function of response regulator output domains. Galperin MY. Curr Opin Microbiol 13, 150-9, (2010). PMID: 20226724

6.The HupR receiver domain crystal structure in its nonphospho and inhibitory phospho states. Davies KM, Lowe ED, Venien-Bryan C, Johnson LN. J. Mol. Biol. 385, 51-64, (2009). View articlePMID: 18977359

7.Identification of a potential transcriptional regulator of hydrogenase activity in free-living Bradyrhizobium japonicum strains. Van Soom C, Verreth C, Sampaio MJ, Vanderleyden J. Mol Gen Genet 239, 235-40, (1993). PMID: 8510650

8.Structural and functional studies of the response regulator HupR. Davies KM, Skamnaki V, Johnson LN, Venien-Bryan C. J Mol Biol 359, 276-88, (2006). PMID: 16631791

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