cd20762

Cap-0 specific (nucleoside-2'-O-)-methyltransferase of nidovirales

CDD entry
Member databaseCDD
CDD typedomain
Short namecapping_2-OMTase_Nidovirales
Setcapping_2-OMTase_viral

Description

Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Nidovirales, a family of ss(+)RNA viruses, cap their mRNAs. For one member, Coronavirus, the 2'OMTase activity is located in the nonstructural protein 16 (NSP16). For others, the 2'OMTase activity may be located in replicase polyprotein 1ab.
[4, 1, 2, 6, 5, 7, 3]

References

1.Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2'-O-methyltransferase nsp10/nsp16 complex. Decroly E, Debarnot C, Ferron F, Bouvet M, Coutard B, Imbert I, Gluais L, Papageorgiou N, Sharff A, Bricogne G, Ortiz-Lombardia M, Lescar J, Canard B. PLoS Pathog. 7, e1002059, (2011). View articlePMID: 21637813

2.Coronavirus non-structural protein 16: evasion, attenuation, and possible treatments. Menachery VD, Debbink K, Baric RS. Virus Res 194, 191-9, (2014). PMID: 25278144

3.The viral RNA capping machinery as a target for antiviral drugs. Ferron F, Decroly E, Selisko B, Canard B. Antiviral Res 96, 21-31, (2012). PMID: 22841701

4.Conventional and unconventional mechanisms for capping viral mRNA. Decroly E, Ferron F, Lescar J, Canard B. Nat Rev Microbiol 10, 51-65, (2011). PMID: 22138959

5.The Nonstructural Proteins Directing Coronavirus RNA Synthesis and Processing. Snijder EJ, Decroly E, Ziebuhr J. Adv. Virus Res. 96, 59-126, (2016). PMID: 27712628

6.Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex. Chen Y, Su C, Ke M, Jin X, Xu L, Zhang Z, Wu A, Sun Y, Yang Z, Tien P, Ahola T, Liang Y, Liu X, Guo D. PLoS Pathog 7, e1002294, (2011). PMID: 22022266

7.mRNA capping: biological functions and applications. Ramanathan A, Robb GB, Chan SH. Nucleic Acids Res 44, 7511-26, (2016). PMID: 27317694

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