cd21517

betacoronavirus non-structural protein 2 (Nsp2) similar to MERS-CoV Nsp2, and related proteins from betacoronaviruses in the C lineage

CDD entry
Member databaseCDD
CDD typedomain
Short namebetaCoV_Nsp2_MERS-like
Setalpha_betaCoV_Nsp2

Description

Coronavirus non-structural proteins (Nsps) are encoded in ORF1a and ORF1b. Post infection, the genomic RNA is released into the cytoplasm of the cell and translated into two long polyproteins (pp), pp1a and pp1ab, which are then autoproteolytically cleaved by two viral proteases Nsp3 and Nsp5 into smaller subunits. Nsp2 is one of these subunits. This subgroup includes Nsp2 from Middle East respiratory syndrome-related coronavirus (MERS-CoV) and betacoronaviruses in the merbecovirus subgenus (C lineage). It belongs to a family which includes Severe acute respiratory syndrome coronavirus (SARS-CoV) Nsp2, and Murine hepatitis virus (MHV) Nsp2 (also known as p65). The function of Nsp2 remains unclear. SARS-CoV Nsp2, rather than playing a role in viral replication, may be involved in altering the host cell environment; deletion of Nsp2 from the SARS-CoV genome results in only a modest reduction in viral titers. It has been shown to interact with two host proteins, prohibitin 1 (PHB1) and PHB2, which have been implicated in cellular functions, including cell-cycle progression, cell migration, cellular differentiation, apoptosis, and mitochondrial biogenesis. MHV Nsp2/p65, different from SARS-CoV Nsp2, may play an important role in the viral life cycle.
[6, 7, 4, 5, 3, 1, 2]

References

1.Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. Woo PC, Lau SK, Chu CM, Chan KH, Tsoi HW, Huang Y, Wong BH, Poon RW, Cai JJ, Luk WK, Poon LL, Wong SS, Guan Y, Peiris JS, Yuen KY. J Virol 79, 884-95, (2005). PMID: 15613317

2.COVID-19 Outbreak: An Overview. Ciotti M, Angeletti S, Minieri M, Giovannetti M, Benvenuto D, Pascarella S, Sagnelli C, Bianchi M, Bernardini S, Ciccozzi M. Chemotherapy 64, 215-223, (2019). PMID: 32259829

3.Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. van Boheemen S, de Graaf M, Lauber C, Bestebroer TM, Raj VS, Zaki AM, Osterhaus AD, Haagmans BL, Gorbalenya AE, Snijder EJ, Fouchier RA. mBio 3, (2012). PMID: 23170002

4.The nsp2 proteins of mouse hepatitis virus and SARS coronavirus are dispensable for viral replication. Graham RL, Sims AC, Baric RS, Denison MR. Adv Exp Med Biol 581, 67-72, (2006). PMID: 17037506

5.A newly identified MHV-A59 ORF1a polypeptide p65 is temperature sensitive in two RNA negative mutants. Hughes SA, Denison MR, Bonilla P, Leibowitz JL, Baric RS, Weiss SR. Adv Exp Med Biol 342, 221-6, (1993). PMID: 8209734

6.Atlas of coronavirus replicase structure. Neuman BW, Chamberlain P, Bowden F, Joseph J. Virus Res 194, 49-66, (2014). PMID: 24355834

7.The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication. Graham RL, Sims AC, Brockway SM, Baric RS, Denison MR. J Virol 79, 13399-411, (2005). PMID: 16227261

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