cd15799

plant invertase/pectin methylesterase inhibitor domain-containing protein

CDD entry
Member databaseCDD
CDD typedomain
Short namePMEI-like_4
SetPMEI_like

Description

This subfamily contains inhibitors similar to those of pectin methylesterase (PME; Pectinesterase; EC 3.1.1.11; CAZy class 8 of carbohydrate esterases) that catalyzes the demethylesterification of homogalacturonans in the cell wall, and cell-wall invertases (CWIs) that catalyze the hydrolytic cleavage of the disaccharide sucrose into glucose and fructose. The proteinaceous PME inhibitor (PMEI) inhibits PME and invertase through formation of a non-covalent 1:1 complex. Cell-wall inhibitor of beta-fructosidase from tobacco (CIF) interacts with CWI in a strictly pH-dependent manner, modulated between pH 4 and 6, with rapid dissociation at neutral pH mediated by structure rearrangement or surface charge pattern in the binding interface. Comparison of the CIF/INV1 structure with the complex between the structurally CIF-related pectin methylesterase inhibitor (PMEI) and pectin methylesterase indicates a common targeting mechanism in PMEI and CIF.
[2, 4, 3, 1]

References

1.Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein. Hothorn M, Van den Ende W, Lammens W, Rybin V, Scheffzek K. Proc. Natl. Acad. Sci. U.S.A. 107, 17427-32, (2010). View articlePMID: 20858733

2.A glycoprotein inhibitor of pectin methylesterase in kiwi fruit. Purification by affinity chromatography and evidence of a ripening-related precursor. Giovane A, Balestrieri C, Quagliuolo L, Castaldo D, Servillo L. Eur. J. Biochem. 233, 926-9, (1995). View articlePMID: 8521860

3.Kiwi protein inhibitor of pectin methylesterase amino-acid sequence and structural importance of two disulfide bridges. Camardella L, Carratore V, Ciardiello MA, Servillo L, Balestrieri C, Giovane A. Eur. J. Biochem. 267, 4561-5, (2000). View articlePMID: 10880981

4.Pectin methylesterase and its proteinaceous inhibitor: a review. Jolie RP, Duvetter T, Van Loey AM, Hendrickx ME. Carbohydr. Res. 345, 2583-95, (2010). View articlePMID: 21047623

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