MF_00250

DNA-directed RNA polymerase subunit Rpo10 [rpo10]

HAMAP entry
Member databaseHAMAP
HAMAP typefamily
Short nameRNApol_arch_Rpo10

Description
Imported from IPR000268

In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (
2.7.7.6
) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consists of an oligomeric assemblage of 10 to 13 polypeptides. Archaebacterial subunit Rpo10 (gene rpoN)
[3]
is a small protein of about 8kDa, it is evolutionary related
[4]
to a 8.3kDa component shared by all three forms of eukaryotic RNA polymerases (RPABC5, gene Rpb10 in yeast and POLR2J in mammals) as well as to African swine fever virus (ASFV) protein CP80R
[2]
.

This family includes both archaeal subunit N, also known as Rpo10 following the eukaryotic nomenclature
[5]
, and eukaryotic Rpb10. There is a conserved region which is located at the N-terminal extremity of these polymerase subunits; this region contains two cysteines that bind a zinc ion
[1]
.

References
Imported from IPR000268

1.Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. Mackereth CD, Arrowsmith CH, Edwards AM, McIntosh LP. Proc. Natl. Acad. Sci. U.S.A. 97, 6316-21, (2000). View articlePMID: 10841539

2.Analysis of the complete nucleotide sequence of African swine fever virus. Yanez RJ, Rodriguez JM, Nogal ML, Yuste L, Enriquez C, Rodriguez JF, Vinuela E. Virology 208, 249-78, (1995). View articlePMID: 11831707

3.Transcription in archaea: similarity to that in eucarya. Langer D, Hain J, Thuriaux P, Zillig W. Proc. Natl. Acad. Sci. U.S.A. 92, 5768-72, (1995). View articlePMID: 7597027

4.Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III. McKune K, Woychik NA. J. Bacteriol. 176, 4754-6, (1994). View articlePMID: 8045907

5.Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure. Korkhin Y, Unligil UM, Littlefield O, Nelson PJ, Stuart DI, Sigler PB, Bell SD, Abrescia NG. PLoS Biol. 7, e102, (2009). View articlePMID: 19419240

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