MF_01509

Replication factor C small subunit [rfcS]

HAMAP entry
Member databaseHAMAP
HAMAP typefamily
Short nameRfcS

Description
Imported from IPR023748

Replication factor C (RFC) is a multimeric AAA+ protein complex that loads the DNA polymerase processivity clamp PCNA (Proliferating Cell Nuclear Antigen) onto DNA using ATP to drive the reaction
[4]
. PCNA functions at multiple levels in directing DNA metabolic pathways
[3]
. When bound to DNA, PCNA organises various proteins involved in DNA replication, DNA repair, DNA modification, and chromatin modelling.

In contrast to eukaryotic RFC, which has been shown to contain five distinct polypeptides, archaeal RFC contains only two types of subunit - large and small. The exact subunit stoichiometry in archaea is unclear. In Methanobacterium thermoautotrophicum, RFC is reported to be a heterohexamer, composed of four small and two large subunits
[2]
. In Sulfolobus solfataricus, meanwhile, it is reported to be a heteropentamer of four small subunits and one large
[1]
.

This entry represents the archaeal RFC small subunit.

References
Imported from IPR023748

1.Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Seybert A, Scott DJ, Scaife S, Singleton MR, Wigley DB. Nucleic Acids Res. 30, 4329-38, (2002). View articlePMID: 12384579

2.A unique organization of the protein subunits of the DNA polymerase clamp loader in the archaeon Methanobacterium thermoautotrophicum deltaH. Kelman Z, Hurwitz J. J. Biol. Chem. 275, 7327-36, (2000). View articlePMID: 10702304

3.The PCNA-RFC families of DNA clamps and clamp loaders. Majka J, Burgers PM. Prog. Nucleic Acid Res. Mol. Biol. 78, 227-60, (2004). View articlePMID: 15210332

4.The replication factor C clamp loader requires arginine finger sensors to drive DNA binding and proliferating cell nuclear antigen loading. Johnson A, Yao NY, Bowman GD, Kuriyan J, O'Donnell M. J. Biol. Chem. 281, 35531-43, (2006). View articlePMID: 16980295

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