F
IPR038837

tRNA ligase 1

InterPro entry
Short nametRNA_ligase_1

Description

tRNA ligase 1 (RNL) is involved in the pre-tRNA splicing process in plants. Pre-tRNA splicing requires intron removal by an endonuclease, and a ligase for joining the resulting tRNA halves. In Arabidopsis, the endonuclease is encoded by two genes, SEN1 and SEN2
[3]
, and tRNA ligase is encoded by one gene, AtRNL
[1]
. Like fungal tRNA ligases, a plant tRNA ligase is a multifunctional enzyme consisting of three domains each with a different activity in the order: ligase, kinase and cyclic phosphodiesterase (CPDase). The polynucleotide kinase domain heals the broken ends to generate the 3'-OH, 2'-PO4, and 5'-PO4 required for sealing by the ligase domain
[2]
.

References

1.Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins. Englert M, Beier H. Nucleic Acids Res. 33, 388-99, (2005). View articlePMID: 15653639

2.Distinctive kinetics and substrate specificities of plant and fungal tRNA ligases. Remus BS, Shuman S. RNA 20, 462-73, (2014). View articlePMID: 24554441

3.Identification of two catalytic subunits of tRNA splicing endonuclease from Arabidopsis thaliana. Akama K, Junker V, Beier H. Gene 257, 177-85, (2000). View articlePMID: 11080584

GO terms

Cross References

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