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Member database | NCBIfam (includes TIGRFAMs) |
NCBIfam type | family |
Short name | RNase_HI-like AI |
Description
AI-generatedUnreviewed
This family of proteins includes nucleases that specialize in degrading the RNA component of RNA-DNA hybrids. They play a crucial role in DNA replication by endonucleolytically removing RNA primers from Okazaki fragments on the lagging strand. These enzymes can function independently but also exhibit activity in the presence of divalent metal ions such as manganese or cobalt, where they can cleave RNA-RNA hybrids, albeit with significantly lower efficiency compared to their primary RNase H activity. They are essential for maintaining genomic stability and can rescue certain bacterial mutants deficient in RNase H function.
References
1. Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7. Ohtani N, Yanagawa H, Tomita M, Itaya M. Nucleic Acids Res 32, 5809-19, (2004). PMID: 15520465
2. Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag. Takano K, Okamoto T, Okada J, Tanaka S, Angkawidjaja C, Koga Y, Kanaya S. PLoS ONE 6, e16226, (2011). View articlePMID: 21283826
Integrated to
External Links
Representative structure
3aly: Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion