TIGR01368

glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit

NCBIfam entry
Member databaseNCBIfam (includes TIGRFAMs)
NCBIfam typefamily
Short nameCPSaseIIsmall

Description

Catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways. This HMM represents the whole of the small chain of the glutamine-dependent form (EC 6.3.5.5) of carbamoyl phosphate synthase, CPSase II. The C-terminal domain has glutamine amidotransferase activity. Note that the sequence from the mammalian urea cycle form has lost the active site Cys, resulting in an ammonia-dependent form, CPSase I (EC 6.3.4.16). CPSases of pyrimidine biosynthesis, arginine biosynthesis, and the urea cycle may be encoded by one or by several genes, depending on the species.

References

1. Inactivation of the amidotransferase activity of carbamoyl phosphate synthetase by the antibiotic acivicin. Miles BW, Thoden JB, Holden HM, Raushel FM. J. Biol. Chem. 277, 4368-73, (2002). View articlePMID: 11729189

2. The structure of carbamoyl phosphate synthetase determined to 2.1 A resolution. Thoden JB, Raushel FM, Benning MM, Rayment I, Holden HM. Acta Crystallogr. D Biol. Crystallogr. 55, 8-24, (1999). View articlePMID: 10089390

3. Structure of carbamoyl phosphate synthetase: a journey of 96 A from substrate to product. Thoden JB, Holden HM, Wesenberg G, Raushel FM, Rayment I. Biochemistry 36, 6305-16, (1997). View articlePMID: 9174345

4. Long-range allosteric transitions in carbamoyl phosphate synthetase. Thoden JB, Huang X, Kim J, Raushel FM, Holden HM. Protein Sci. 13, 2398-405, (2004). View articlePMID: 15322282

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