PTHR11630

DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER

PANTHER entry
Member databasePANTHER
PANTHER typefamily

Description
Imported from IPR031327

MCM proteins are DNA-dependent ATPases required for the initiation of eukaryotic DNA replication
[4, 7, 3]
. In eukaryotes there is a family of eight proteins, MCM2 to MCM9. They were first identified in yeast where most of them have a direct role in the initiation of chromosomal DNA replication by interacting directly with autonomously replicating sequences (ARS). They were thus called minichromosome maintenance proteins, MCM proteins
[8]
. These proteins are evolutionarily related and belong to the AAA+ superfamily. They contain the Mcm family domain, which includes motifs that are required for ATP hydrolysis (such as the Walker A and B, and R-finger motifs). Mcm2-7 forms a hexameric complex
[6]
in which individual subunits associate with different affinities, and there is a tightly associated core of Mcm4 (Cdc21), Mcm6 (Mis5) and Mcm7
[5]
. Mcm2-7 complex is the replicative helicase involved in replication initiation and elongation
[2]
, whereas Mcm8 and Mcm9 from and separate one, conserved among many eukaryotes except yeast and C. elegans. Mcm8/9 complex play a role during replication elongation or recombination, being involved in the repair of double-stranded DNA breaks and DNA interstrand cross-links by homologous recombination. Drosophila is the only organism that has MCM8 without MCM9, involved in meiotic recombination
[10, 9]
.

This family is also present in the archebacteria in 1 to 4 copies. Methanocaldococcus jannaschii (Methanococcus jannaschii) has four members, MJ0363, MJ0961, MJ1489 and MJECL13.

Schizosaccharomyces pombe (Fission yeast) MCMs, like those in metazoans, are found in the nucleus throughout the cell cycle. This is in contrast to the Saccharomyces cerevisiae (Baker's yeast) in which MCM proteins move in and out of the nucleus during each cell cycle. The assembly of the MCM complex in S. pombe is required for MCM localisation, ensuring that only intact MCM complexes remain in the nucleus
[1]
.

References
Imported from IPR031327

1.Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly. Pasion SG, Forsburg SL. Mol. Biol. Cell 10, 4043-57, (1999). View articlePMID: 10588642

2.A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. Ishimi Y. J. Biol. Chem. 272, 24508-13, (1997). View articlePMID: 9305914

3.The MCM2-3-5 proteins: are they replication licensing factors? Tye BK. Trends Cell Biol. 4, 160-6, (1994). View articlePMID: 14731643

4.Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication. Coxon A, Maundrell K, Kearsey SE. Nucleic Acids Res. 20, 5571-7, (1992). View articlePMID: 1454522

5.Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex. Sherman DA, Pasion SG, Forsburg SL. Mol. Biol. Cell 9, 1833-45, (1998). View articlePMID: 9658174

6.A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast. Adachi Y, Usukura J, Yanagida M. Genes Cells 2, 467-79, (1997). View articlePMID: 9366552

7.The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. Hu B, Burkhart R, Schulte D, Musahl C, Knippers R. Nucleic Acids Res. 21, 5289-93, (1993). View articlePMID: 8265339

8.A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Koonin EV. Nucleic Acids Res. 21, 2541-7, (1993). View articlePMID: 8332451

9.The MCM8/9 complex: A recent recruit to the roster of helicases involved in genome maintenance. Griffin WC, Trakselis MA. DNA Repair (Amst.) 76, 1-10, (2019). PMID: 30743181

10.Mcm8 and Mcm9 form a complex that functions in homologous recombination repair induced by DNA interstrand crosslinks. Nishimura K, Ishiai M, Horikawa K, Fukagawa T, Takata M, Takisawa H, Kanemaki MT. Mol. Cell 47, 511-22, (2012). PMID: 22771115

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