PTHR22976

BIOTIN SYNTHASE

PANTHER entry
Member databasePANTHER
PANTHER typefamily

Description
Imported from IPR002684

This family consists of biotin synthase B-like and bifunctional biotin synthase AB proteins.

Biotin synthase
2.8.1.6
works with flavodoxin, S-adenosylmethionine, and possibly cysteine to catalyze the last step of the biotin biosynthetic pathway. The reaction consists of the introduction of a sulphur atom into dethiobiotin, thus requiring activation of C-H bonds
[2]
. Biotin (vitamin H) is a prosthetic group in enzymes catalysing carboxylation and transcarboxylation reactions
[3]
.

Biotin synthase from Escherichia coli is a homodimer of 76kDa, with each polypeptide chain carrying an oxygen-sensitive (4Fe-4S) cluster, probably ligated by three cysteines of a CXXXCXXC box conserved among all known BioB sequences and a fourth still not identified ligand. BioB displays a pyridoxal phosphate-dependent cysteine desulphurase activity, which allows mobilisation of the sulphur atom from free cysteine
[1]
.

References
Imported from IPR002684

1.Biotin synthase is a pyridoxal phosphate-dependent cysteine desulfurase. Ollagnier-De-Choudens S, Mulliez E, Hewitson KS, Fontecave M. Biochemistry 41, 9145-52, (2002). View articlePMID: 12119030

2.Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. Bower S, Perkins JB, Yocum RR, Howitt CL, Rahaim P, Pero J. J. Bacteriol. 178, 4122-30, (1996). View articlePMID: 8763940

3.Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum. Serebriiskii IG, Vassin VM, Tsygankov YD. Gene 175, 15-22, (1996). View articlePMID: 8917070

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