Member database | PANTHER |
PANTHER type | family |
Description Imported from IPR002684
This family consists of biotin synthase B-like and bifunctional biotin synthase AB proteins.
Biotin synthase
2.8.1.6 works with flavodoxin, S-adenosylmethionine, and possibly cysteine to catalyze the last step of the biotin biosynthetic pathway. The reaction consists of the introduction of a sulphur atom into dethiobiotin, thus requiring activation of C-H bonds
[2]. Biotin (vitamin H) is a prosthetic group in enzymes catalysing carboxylation and transcarboxylation reactions
[3].
Biotin synthase from Escherichia coli is a homodimer of 76kDa, with each polypeptide chain carrying an oxygen-sensitive (4Fe-4S) cluster, probably ligated by three cysteines of a CXXXCXXC box conserved among all known BioB sequences and a fourth still not identified ligand. BioB displays a pyridoxal phosphate-dependent cysteine desulphurase activity, which allows mobilisation of the sulphur atom from free cysteine
[1].
References Imported from IPR002684
1.Biotin synthase is a pyridoxal phosphate-dependent cysteine desulfurase. Ollagnier-De-Choudens S, Mulliez E, Hewitson KS, Fontecave M. Biochemistry 41, 9145-52, (2002). View articlePMID: 12119030
2.Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. Bower S, Perkins JB, Yocum RR, Howitt CL, Rahaim P, Pero J. J. Bacteriol. 178, 4122-30, (1996). View articlePMID: 8763940
3.Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum. Serebriiskii IG, Vassin VM, Tsygankov YD. Gene 175, 15-22, (1996). View articlePMID: 8917070
Integrated to
External Links
Representative structure
8vcw: X-Ray Crystal Structure of the biotin synthase from B. obeum