PTHR43213

BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED

PANTHER entry
Member databasePANTHER
PANTHER typefamily

Description
Imported from IPR003697

The Maf protein of Bacillus subtilis shares substantial amino acid sequence identity with Escherichia coli YhdE (previously known as OrfE)
[1]
. Maf-like proteins are conserved in bacteria, archaea, and eukaryotes. Maf proteins have been implicated in cell division arrest
[3]
. It has also been proposed that they belong to a family of house-cleaning nucleotide hydrolyzing enzymes which prevent the incorporation of noncanonical nucleotides into cellular DNA
[4]
. Maf proteins exhibit nucleotide pyrophosphatase activity against canonical and modified nucleotides, which might represent a molecular mechanism for this dual role in cell division arrest and in house-cleaning
[2]
.

This entry includes pyrophosphatases, such as YhdE and YceF from E. coli. YhdE is a nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP
[2, 5, 6]
. YceF is a nucleoside triphosphate pyrophosphatase that hydrolyzes 7-methyl-GTP (m7GTP)
[7]
.

References
Imported from IPR003697

1.Amplification of the Bacillus subtilis maf gene results in arrested septum formation. Butler YX, Abhayawardhane Y, Stewart GC. J. Bacteriol. 175, 3139-45, (1993). View articlePMID: 8387996

2.Biochemical and structural studies of conserved Maf proteins revealed nucleotide pyrophosphatases with a preference for modified nucleotides. Tchigvintsev A, Tchigvintsev D, Flick R, Popovic A, Dong A, Xu X, Brown G, Lu W, Wu H, Cui H, Dombrowski L, Joo JC, Beloglazova N, Min J, Savchenko A, Caudy AA, Rabinowitz JD, Murzin AG, Yakunin AF. Chem. Biol. 20, 1386-98, (2013). View articlePMID: 24210219

3.Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis. Briley K Jr, Prepiak P, Dias MJ, Hahn J, Dubnau D. Mol. Microbiol. 81, 23-39, (2011). View articlePMID: 21564336

4.House cleaning, a part of good housekeeping. Galperin MY, Moroz OV, Wilson KS, Murzin AG. Mol. Microbiol. 59, 5-19, (2006). View articlePMID: 16359314

5.Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli. Jin J, Wu R, Zhu J, Yang S, Lei Z, Wang N, Singh VK, Zheng J, Jia Z. PLoS ONE 10, e0117823, (2015). View articlePMID: 25658941

6.A new method to investigate the catalytic mechanism of YhdE pyrophosphatase by using a pyrophosphate fluorescence probe. Shen Q, Tan H, Xing GW, Zheng J, Jia Z. Sci Rep 7, 8169, (2017). View articlePMID: 28811554

7.[Valve of the detection of Australia antigen in blood donors] Medarde Agustin A, Aranaz Iriberri L, De Galarreta LA, Dianda CA, Madoz Resano P. Rev Sanid Hig Publica (Madr) 47, 529-36, (1973). PMID: 4210219

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