PF00814

tRNA N6-adenosine threonylcarbamoyltransferase

Pfam entry
Member databasePfam
Pfam typefamily
Short nameTsaD
ClanActin_ATPase
Author El-Gebali S;0000-0003-1378-5495 Bateman A;0000-0002-6982-4660
Sequence Ontology0100021

Description

This domain can be found in Kae1/Qri7/YgjD, products of COG0533 that belong to a small group of 60 proteins that are present in all three domains of life. COG0533 proteins are suggest to play a role in a post translational modification of certain tRNAs. For example, YgjD along with YeaZ, YjeE, and YrdC have been deemed necessary and sufficient for the tRNA modification
[2]
. This modification involves the formation of N6-threonyl carbamoyl adenosine (t6A) at position 37 in the anti-codon stem loop which is critical for translational speed and accuracy
[1]
. Structural analysis indicate that YeaZ lacks resemblance to any known protease active site. Together with the absence of a putative zinc-binding motif. Thus the likelyhood of it being a protease, as previously thought, has been negated
[2]
. EC:2.3.1.234

References

1.Global translational impacts of the loss of the tRNA modification t<sup>6</sup>A in yeast. Thiaville PC, Legendre R, Rojas-Benitez D, Baudin-Baillieu A, Hatin I, Chalancon G, Glavic A, Namy O, de Crecy-Lagard V. Microb Cell 3, 29-45, (2016). PMID: 26798630

2.Crystal structure of the dimer of two essential Salmonella typhimurium proteins, YgjD & YeaZ and calorimetric evidence for the formation of a ternary YgjD-YeaZ-YjeE complex. Nichols CE, Lamb HK, Thompson P, Omari KE, Lockyer M, Charles I, Hawkins AR, Stammers DK. Protein Sci. (2013). View articlePMID: 23471679

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