PF01609

Transposase DDE domain

Pfam entry
Member databasePfam
Pfam typedomain
Short nameDDE_Tnp_1
ClanRNase_H
Author Bateman A;0000-0002-6982-4660
Sequence Ontology0000417

Description

Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction
[4]
. This family contains transposases for IS4 Swiss:P03835
[2]
, IS421 Swiss:P11901
[5]
, IS5377 Swiss:Q45620, IS427
[1]
, IS402
[6]
, IS1355 Swiss:O69604, IS5, which was original isolated in bacteriophage lambda
[3]
.

References

1.The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution. Davies DR, Mahnke Braam L, Reznikoff WS, Rayment I. J. Biol. Chem. 274, 11904-13, (1999). View articlePMID: 10207011

2.The sequence of IS4. Klaer R, Kuhn S, Tillmann E, Fritz HJ, Starlinger P. Mol. Gen. Genet. 181, 169-75, (1981). View articlePMID: 6268937

3.Nucleotide sequence of IS402 from Pseudomonas cepacia. Ferrante AA, Lessie TG. Gene 102, 143-4, (1991). View articlePMID: 1650732

4.Identification and analysis of the gas vesicle gene cluster on an unstable plasmid of Halobacterium halobium. DasSarma S. Experientia 49, 482-6, (1993). View articlePMID: 8335077

5.IS421, a new insertion sequence in Escherichia coli. Sato S, Nakada Y, Shiratsuchi A. FEBS Lett. 249, 21-6, (1989). View articlePMID: 2542093

6.Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden J. Plasmid 24, 227-34, (1990). View articlePMID: 1963949

Further reading

7. Structural analysis of insertion sequence IS5. Kroger M, Hobom G. Nature 297, 159-62, (1982). View articlePMID: 6281654

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