PF01612

3'-5' exonuclease

Pfam entry
Member databasePfam
Pfam typedomain
Short nameDNA_pol_A_exo1
ClanRNase_H
Author Bashton M;0000-0002-6847-1525 Bateman A;0000-0002-6982-4660 Griffiths-Jones SR;0000-0001-6043-807X
Sequence Ontology0000417

Description

This domain is responsible for the 3'-5' exonuclease proofreading activity of E. coli DNA polymerase I (polI) and other enzymes, it catalyses the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli polI it is also found in the Werner syndrome helicase (WRN), focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D). Werner syndrome is a human genetic disorder causing premature aging; the WRN protein has helicase activity in the 3'-5' direction
[3, 1]
. The FFA-1 protein is required for formation of a replication foci and also has helicase activity; it is a homologue of the WRN protein
[2]
. RNase D is a 3'-5' exonuclease involved in tRNA processing. Also found in this family is the autoantigen PM/Scl thought to be involved in polymyositis-scleroderma overlap syndrome.

References

1.DNA helicase activity in Werner's syndrome gene product synthesized in a baculovirus system. Suzuki N, Shimamoto A, Imamura O, Kuromitsu J, Kitao S, Goto M, Furuichi Y. Nucleic Acids Res. 25, 2973-8, (1997). View articlePMID: 9224595

2.Replication focus-forming activity 1 and the Werner syndrome gene product. Yan H, Chen CY, Kobayashi R, Newport J. Nat. Genet. 19, 375-8, (1998). View articlePMID: 9697700

3.The Werner syndrome protein is a DNA helicase. Gray MD, Shen JC, Kamath-Loeb AS, Blank A, Sopher BL, Martin GM, Oshima J, Loeb LA. Nat. Genet. 17, 100-3, (1997). View articlePMID: 9288107

Further reading

4. The proofreading domain of Escherichia coli DNA polymerase I and other DNA and/or RNA exonuclease domains. Moser MJ, Holley WR, Chatterjee A, Mian IS. Nucleic Acids Res. 25, 5110-8, (1997). View articlePMID: 9396823

5. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP. Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA. Nature 313, 762-6, (1985). View articlePMID: 3883192

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