PF03063

Prismane/CO dehydrogenase family

Pfam entry
Member databasePfam
Pfam typefamily
Short namePrismane
Author Griffiths-Jones SR;0000-0001-6043-807X Bateman A;0000-0002-6982-4660
Sequence Ontology0100021

Description

This family includes both hybrid-cluster proteins and the beta chain of carbon monoxide dehydrogenase. The hybrid-cluster proteins contain two Fe/S centres - a [4Fe-4S] cubane cluster, and a hybrid [4Fe-2S-2O] cluster. The physiological role of this protein is as yet unknown, although a role in nitrate/nitrite respiration has been suggested
[2]
. The prismane protein from Escherichia coli was shown to contain hydroxylamine reductase activity (NH2OH + 2e + 2 H+ -> NH3 + H2O). This activity is rather low. Hydroxylamine reductase activity was also found in CO-dehydrogenase in which the active site Ni was replaced by Fe
[1]
. The CO dehydrogenase contains a Ni-3Fe-2S-3O centre.

References

1.Hydroxylamine reductase activity of the hybrid cluster protein from Escherichia coli. Wolfe MT, Heo J, Garavelli JS, Ludden PW. J. Bacteriol. 184, 5898-902, (2002). View articlePMID: 12374823

2.The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S]. van den Berg WA, Hagen WR, van Dongen WM. Eur. J. Biochem. 267, 666-76, (2000). View articlePMID: 10651802

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