PF14480

DNA polymerase III polC-type N-terminus I

Pfam entry
Member databasePfam
Pfam typefamily
Short nameDNA_pol3_a_NI
Author Eberhardt R;0000-0001-6152-1369
Sequence Ontology0100021

Description

This is the first N-terminal domain, NI domain, of the DNA polymerase III polC subunit A that is found only in Firmicutes. DNA polymerase polC-type III enzyme functions as the 'replicase' in low G + C Gram-positive bacteria
[1]
. Purine asymmetry is a characteristic of organisms with a heterodimeric DNA polymerase III alpha-subunit constituted by polC which probably plays a direct role in the maintenance of strand-biased gene distribution; since, among prokaryotic genomes, the distribution of genes on the leading and lagging strands of the replication fork is known to be biased
[2]
. It has been predicted that the N-terminus of polC folds into two globular domains, NI and NII. A predicted patch of elecrostatic potential at the surface of this domain suggests a possible involvement in nucleic acid binding
[3]
. This domain is associated with DNA_pol3_alpha Pfam:PF07733 and DNA_pol3_a_NI Pfam:PF11490.

References

1.A 'gram-negative-type' DNA polymerase III is essential for replication of the linear chromosome of Streptomyces coelicolor A3(2). Flett F, de Mello Jungmann-Campello D, Mersinias V, Koh SL, Godden R, Smith CP. Mol. Microbiol. 31, 949-58, (1999). View articlePMID: 10048037

2.Replication-associated purine asymmetry may contribute to strand-biased gene distribution. Hu J, Zhao X, Yu J. Genomics 90, 186-94, (2007). View articlePMID: 17532183

3.The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III τ subunit. Timinskas K, Venclovas C. FEBS J. 278, 3109-18, (2011). View articlePMID: 21740522

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