PF17289

Terminase RNaseH-like domain

Pfam entry
Member databasePfam
Pfam typedomain
Short nameTerminase_6C
ClanRNase_H
Author Bateman A;0000-0002-6982-4660
Sequence Ontology0000417

Description
Imported from IPR035421

This entry represents the C-terminal domain found in the terminase large subunits. Terminase is a component of the molecular motor that translocates genomic DNA into empty capsids during DNA packaging
[1]
. The large subunit heterodimerises with the small terminase protein, which is docked on the capsid portal protein. The latter forms a ring through which genomic DNA is translocated into the capsid. The terminase protein has endonuclease activity to cleave DNA after encapsidation
[5, 6]
.

Proteins containing this domain include gp17 from bacteriophage T4 (
P17312
)
[3, 2, 4]
.

References
Imported from IPR035421

1.DNA packaging in dsDNA bacteriophages. Black LW. Annu. Rev. Microbiol. 43, 267-92, (1989). View articlePMID: 2679356

2.Sequence analysis of bacteriophage T4 DNA packaging/terminase genes 16 and 17 reveals a common ATPase center in the large subunit of viral terminases. Mitchell MS, Matsuzaki S, Imai S, Rao VB. Nucleic Acids Res. 30, 4009-21, (2002). View articlePMID: 12235385

3.Structural analysis of DNA cleaved in vivo by bacteriophage T4 terminase. Bhattacharyya SP, Rao VB. Gene 146, 67-72, (1994). View articlePMID: 8063105

4.Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity. Baumann RG, Black LW. J Biol Chem 278, 4618-27, (2003). PMID: 12466275

5.A viral small terminase subunit (TerS) twin ring pac synapsis DNA packaging model is supported by fluorescent fusion proteins. Dixit AB, Ray K, Black LW. Virology 536, 39-48, (2019). PMID: 31400548

6.Small terminase couples viral DNA binding to genome-packaging ATPase activity. Roy A, Bhardwaj A, Datta P, Lander GC, Cingolani G. Structure 20, 1403-13, (2012). View articlePMID: 22771211

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.