PF17681

Gamma tubulin complex component N-terminal

Pfam entry
Member databasePfam
Pfam typedomain
Short nameGCP_N_terminal
ClanGCP
Author El-Gebali S;0000-0003-1378-5495
Sequence Ontology0000417

Description

This is the N-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast
[3]
. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated
Q9UGJ1
[1]
. Functional studies have shown that the N-terminal domain defines the functional identity of GCPs, suggesting that all GCPs are incorporated into the helix of gamma-tubulin small complexes (gTURCs) via lateral interactions between their N-terminal domains. Thereby, they define the direct neighbors and position the GCPs within the helical wall of gTuRC
[2]
. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains
[1]
. In addition to the conserved sequences, the N-terminal domains carry specific insertions of various sizes depending on the GCP, i.e. internal insertions or N-terminal extensions. These insertions may equally contribute to the function of individual GCPs as they have been implied in specific interactions with regulatory or structural proteins. For instance, GCP6 carries a large internal insertion phosphorylated by Plk4 and containing a domain of interaction with keratins, whereas the N-terminal extension of GCP3 interacts with the recruitment protein MOZART1
[2]
.

References

1.Crystal structure of γ-tubulin complex protein GCP4 provides insight into microtubule nucleation. Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L. Nat. Struct. Mol. Biol. 18, 915-9, (2011). View articlePMID: 21725292

2.Functional Analysis of γ-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains. Farache D, Jauneau A, Chemin C, Chartrain M, Remy MH, Merdes A, Haren L. J. Biol. Chem. 291, 23112-23125, (2016). View articlePMID: 27660388

3.Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. Gunawardane RN, Martin OC, Cao K, Zhang L, Dej K, Iwamatsu A, Zheng Y. J. Cell Biol. 151, 1513-24, (2000). View articlePMID: 11134079

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.