PF18319

PriA DNA helicase Cys-rich region (CRR) domain

Pfam entry
Member databasePfam
Pfam typedomain
Short namePriA_CRR
ClanZn_Beta_Ribbon
Author El-Gebali S;0000-0003-1378-5495
Sequence Ontology0000417

Description

This is a cys-rich region (CRR) domain found in PriA DNA helicases. In bacteria, the replication restart process is orchestrated by the PriA DNA helicase, which identifies replication forks via structure-specific DNA binding and interactions with fork-associated ssDNA-binding proteins (SSBs). The CRR region which is embedded within the C-terminal helicase lobe has been identified to bind two Zn2+ ions. This 50-residue insertion forms a structure on the surface of the helicase core in which two Zn2+ ions are coordinated by invariant Cys residues. Biochemical experiments have shown that sequence changes to Zn2+-binding Cys residues in the PriA CRR can eliminate helicase, but not ATPase, activity and can block assembly of PriB onto DNA-bound PriA, implicating the CRR in multiple functions in PriA
[1]
.

References

1.Structural mechanisms of PriA-mediated DNA replication restart. Bhattacharyya B, George NP, Thurmes TM, Zhou R, Jani N, Wessel SR, Sandler SJ, Ha T, Keck JL. Proc. Natl. Acad. Sci. U.S.A. 111, 1373-8, (2014). View articlePMID: 24379377

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