Member database | Pfam |
Pfam type | domain |
Short name | IntS14_b-barrel |
Author | Chuguransky S;0000-0002-0520-0736 Bateman A;0000-0002-6982-4660 |
Sequence Ontology | 0000417 |
Description
This entry represents the central beta-barrel domain of Integrator complex subunit 14 (IntS14), a component of the integrator complex which is involved in the transcription of small nuclear RNAs (snRNA) and their 3'-box-dependent processing. This complex is also involved in the 3'-end processing of the U7 snRNA, and the spliceosomal snRNAs U1 and U5
[2]. IntS14 interacts with IntS13, both of them consist of an N-terminal von-Willebrand type A like domain (VWA), followed by a central beta-barrel domain and a C-terminal alpha-helical domain connected through a linker. The interface involves all domains of both proteins, both beta-barrel domains form the centre of the complex and contribute to the major contact between Ints13-IntS14
[1].
References
1.INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module. Sabath K, Staubli ML, Marti S, Leitner A, Moes M, Jonas S. Nat Commun 11, 3422, (2020). PMID: 32647223
2.An RNAi screen identifies additional members of the Drosophila Integrator complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation. Chen J, Ezzeddine N, Waltenspiel B, Albrecht TR, Warren WD, Marzluff WF, Wagner EJ. RNA 18, 2148-56, (2012). View articlePMID: 23097424