PF22600

Poly(A) RNA polymerase, mitochondrial-like, central palm domain

Pfam entry
Member databasePfam
Pfam typedomain
Short nameMTPAP-like_central
ClanNTP_transf
Author Bateman A;0000-0002-6982-4660 Lazaro Pinto Beatriz;0000-0001-6837-2941
Sequence Ontology0000417

Description

This domain is found in human Poly(A) RNA polymerase, mitochondrial (MTPAP) and similar proteins from eukaryotes. MTPAP is a noncanonical polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. It contains three domains: a N-terminal domain RL (Pfam:PF17797) and the canonical palm (this entry) and fingers domains. This domain shows a five -stranded beta-sheet with the three catalytic aspartic acid residues located on its surface
[1]
, together with several alpha- helices, forming an alpha-beta configuration.

References

1.Structural basis for dimerization and activity of human PAPD1, a noncanonical poly(A) polymerase. Bai Y, Srivastava SK, Chang JH, Manley JL, Tong L. Mol. Cell 41, 311-20, (2011). View articlePMID: 21292163

Further reading

2. Crystal structure of mammalian poly(A) polymerase in complex with an analog of ATP. Martin G, Keller W, Doublie S. EMBO J. 19, 4193-203, (2000). View articlePMID: 10944102

3. Structure of HI0073 from Haemophilus influenzae, the nucleotide-binding domain of a two-protein nucleotidyl transferase. Lehmann C, Pullalarevu S, Krajewski W, Willis MA, Galkin A, Howard A, Herzberg O. Proteins 60, 807-11, (2005). View articlePMID: 16028221

4. Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase. Martin G, Moglich A, Keller W, Doublie S. J. Mol. Biol. 341, 911-25, (2004). View articlePMID: 15328606

5. Structure of yeast poly(A) polymerase alone and in complex with 3'-dATP. Bard J, Zhelkovsky AM, Helmling S, Earnest TN, Moore CL, Bohm A. Science 289, 1346-9, (2000). View articlePMID: 10958780

6. Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. Deng J, Ernst NL, Turley S, Stuart KD, Hol WG. EMBO J. 24, 4007-17, (2005). View articlePMID: 16281058

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