PR00008

DAGPEDOMAIN

PRINTS entry
Member databasePRINTS
PRINTS typedomain
Short nameDAGPEDOMAIN

Description
Imported from IPR020454

Diacylglycerol (DAG) is an important second messenger. Phorbol esters (PE) are analogues of DAG and potent tumour promoters that cause a variety of physiological changes when administered to both cells and tissues. DAG activates a family of serine/threonine protein kinases, collectively known as protein kinase C (PKC)
[1]
. Phorbol esters can directly stimulate PKC. The N-terminal region of PKC, known as C1, has been shown
[2]
to bind PE and DAG in a phospholipid and zinc-dependent fashion. The C1 region contains one or two copies (depending on the isozyme of PKC) of a cysteine-rich domain, which is about 50 amino-acid residues long, and which is essential for DAG/PE-binding. The DAG/PE-binding domain binds two zinc ions; the ligands of these metal ions are probably the six cysteines and two histidines that are conserved in this domain.

References
Imported from IPR020454

1.The protein kinase C family. Azzi A, Boscoboinik D, Hensey C. Eur. J. Biochem. 208, 547-57, (1992). View articlePMID: 1396661

2.Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence. Ono Y, Fujii T, Igarashi K, Kuno T, Tanaka C, Kikkawa U, Nishizuka Y. Proc. Natl. Acad. Sci. U.S.A. 86, 4868-71, (1989). View articlePMID: 2500657

Supplementary References

1. Product of vav proto-oncogene defines a new class of tyrosine protein kinase substrates. Bustelo XR, Ledbetter JA, Barbacid M. Nature 356, 68-71, (1992). View articlePMID: 1311423

2. Tyrosine phosphorylation of vav proto-oncogene product containing SH2 domain and transcription factor motifs. Margolis B, Hu P, Katzav S, Li W, Oliver JM, Ullrich A, Weiss A, Schlessinger J. Nature 356, 71-4, (1992). View articlePMID: 1531699

3. The protein kinase C family: heterogeneity and its implications. Kikkawa U, Kishimoto A, Nishizuka Y. Annu. Rev. Biochem. 58, 31-44, (1989). View articlePMID: 2549852

4. Proto-vav and gene expression. Boguski MS, Bairoch A, Attwood TK, Michaels GS. Nature 358, 113, (1992). View articlePMID: 1614545

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