PR00759

BASICPTASE

PRINTS entry
Member databasePRINTS
PRINTS typedomain
Short nameBASICPTASE

Description
Imported from IPR002223

The majority of the sequences having this domain belong to the MEROPS inhibitor family I2, clan IB; the Kunitz/bovine pancreatic trypsin inhibitor family, they inhibit proteases of the S1 family
[6]
and are restricted to the metazoa with a single exception: Amsacta moorei entomopoxvirus. They are short (~50 residue) α/β proteins with few secondary structures. The fold is constrained by 3 disulphide bonds. The type example for this family is aprotinin (bovine pancreatic trypsin inhibitor)
[1]
(or basic protease inhibitor), but the family includes numerous other members
[2, 3, 4, 5]
, such as snake venom basic protease; mammalian inter-alpha-trypsin inhibitors; trypstatin, a rodent mast cell inhibitor of trypsin; a domain found in an alternatively-spliced form of Alzheimer's amyloid beta-protein; domains at the C-termini of the alpha(1) and alpha(3) chains of type VII and type VI collagens; and tissue factor pathway inhibitor precursor.

The pancreatic trypsin inhibitor (Kunitz) family
[7, 2, 3]
is one of the numerous families of serine proteinase inhibitors. The basic structure of such a type of inhibitor is shown in the following schematic representation:

            +-----------------------+
            |  +--------+           |
            |  |      **|*******    |
          xxCxxC#xxxCxxxCxxxxxxCxxxxCxx
                    |          |
                    +----------+

            {------50 residues------}

'C': conserved cysteine involved in a disulfide bond.
'#': active site residue.
'*': position of the pattern.

References
Imported from IPR002223

1.Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor. Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A. J. Mol. Biol. 220, 757-70, (1991). View articlePMID: 1714504

2.Inter-alpha-trypsin inhibitor: emergence of a family within the Kunitz-type protease inhibitor superfamily. Salier JP. Trends Biochem. Sci. 15, 435-9, (1990). View articlePMID: 1703675

3.Evolutionary origin of a Kunitz-type trypsin inhibitor domain inserted in the amyloid beta precursor protein of Alzheimer's disease. Ikeo K, Takahashi K, Gojobori T. J. Mol. Evol. 34, 536-43, (1992). View articlePMID: 1593645

4.Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor. Sprecher CA, Kisiel W, Mathewes S, Foster DC. Proc. Natl. Acad. Sci. U.S.A. 91, 3353-7, (1994). View articlePMID: 8159751

5.Amino acid sequence of a protease inhibitor isolated from Sarcophaga bullata determined by mass spectrometry. Papayannopoulos IA, Biemann K. Protein Sci. 1, 278-88, (1992). View articlePMID: 1304909

6.Evolutionary families of peptidase inhibitors. Rawlings ND, Tolle DP, Barrett AJ. Biochem. J. 378, 705-16, (2004). View articlePMID: 14705960

7.Protein inhibitors of proteinases. Laskowski M Jr, Kato I. Annu. Rev. Biochem. 49, 593-626, (1980). View articlePMID: 6996568

Supplementary References

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