Member database | PRINTS |
PRINTS type | family |
Short name | NUCDPKINASE |
Description Imported from IPR001564
Nucleoside diphosphate kinases (
2.7.4.6) (NDK) are enzymes required for the synthesis of nucleoside triphosphates (NTP) other than ATP. They provide NTPs for nucleic acid synthesis, CTP for lipid synthesis, UTP for polysaccharide synthesis and GTP for protein elongation, signal transduction and microtubule polymerisation.
NDK are proteins of 17 Kd that act via a ping-pong mechanism in which a histidine residue is phosphorylated, by transfer of the terminal phosphate group from ATP. In the presence of magnesium, the phosphoenzyme can transfer its phosphate group to any NDP, to produce an NTP.
NDK isozymes have been sequenced from prokaryotic and eukaryotic sources. It has also been shown
[1] that the Drosophila awd (abnormal wing discs) protein, is a microtubule-associated NDK. Mammalian NDK is also known as metastasis inhibition factor nm23. The sequence of NDK has been highly conserved through evolution. There is a single histidine residue conserved in all known NDK isozymes, which is involved in the catalytic mechanism
[2].
References Imported from IPR001564
1.A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphate kinase. Biggs J, Hersperger E, Steeg PS, Liotta LA, Shearn A. Cell 63, 933-40, (1990). View articlePMID: 2175255
2.Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme. Gilles AM, Presecan E, Vonica A, Lascu I. J. Biol. Chem. 266, 8784-9, (1991). View articlePMID: 1851158
Supplementary References
1. X-ray structure of nucleoside diphosphate kinase. Dumas C, Lascu I, Morera S, Glaser P, Fourme R, Wallet V, Lacombe ML, Veron M, Janin J. EMBO J. 11, 3203-8, (1992). View articlePMID: 1324167
Integrated to
Representative structure
4ane: R80N MUTANT OF NUCLEOSIDE DIPHOSPHATE KINASE FROM MYCOBACTERIUM TUBERCULOSIS