PR01657

MCMFAMILY

PRINTS entry
Member databasePRINTS
PRINTS typefamily
Short nameMCMFAMILY

Description
Imported from IPR001208

Proteins shown to be required for the initiation of eukaryotic DNA replication share a highly conserved domain of about 210 amino-acid residues
[2, 3, 1]
. The latter shows some similarities
[4]
with that of various other families of DNA-dependent ATPases. Eukaryotes seem to possess a family of eight proteins that contain this domain. They were first identified in yeast where most of them have a direct role in the initiation of chromosomal DNA replication by interacting directly with autonomously replicating sequences (ARS). They were thus called 'minichromosome maintenance proteins' with gene symbols prefixed by MCM. These six proteins are:


 * MCM2, also known as cdc19 (in S.pombe).
 * MCM3, also known as DNA polymerase alpha holoenzyme-associated protein P1, RLF beta subunit or ROA.
 * MCM4, also known as CDC54, cdc21 (in S.pombe) or dpa (in Drosophila).
 * MCM5, also known as CDC46 or nda4 (in S.pombe).
 * MCM6, also known as mis5 (in S.pombe).
 * MCM7, also known as CDC47 or Prolifera (in A.thaliana).
 * MCM8, also known as as REC (in Drosophila).
 * MCM


These proteins are evolutionarily related and belong to the AAA+ superfamily. They contain the Mcm family domain, which includes motifs that are required for ATP hydrolysis (such as the Walker A and B, and R-finger motifs). Mcm2-7 forms a hexameric complex which is the replicative helicase involved in replication initiation and elongation, whereas Mcm8 and Mcm9 from and separate one, conserved among many eukaryotes except yeast and C. elegans. Mcm8/9 complex play a role during replication elongation or recombination, being involved in the repair of double-stranded DNA breaks and DNA interstrand cross-links by homologous recombination. Drosophila is the only organism that has MCM8 without MCM9, involved in meiotic recombination
[6, 5]
.

References
Imported from IPR001208

1.The MCM2-3-5 proteins: are they replication licensing factors? Tye BK. Trends Cell Biol. 4, 160-6, (1994). View articlePMID: 14731643

2.Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication. Coxon A, Maundrell K, Kearsey SE. Nucleic Acids Res. 20, 5571-7, (1992). View articlePMID: 1454522

3.The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins. Hu B, Burkhart R, Schulte D, Musahl C, Knippers R. Nucleic Acids Res. 21, 5289-93, (1993). View articlePMID: 8265339

4.A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Koonin EV. Nucleic Acids Res. 21, 2541-7, (1993). View articlePMID: 8332451

5.The MCM8/9 complex: A recent recruit to the roster of helicases involved in genome maintenance. Griffin WC, Trakselis MA. DNA Repair (Amst.) 76, 1-10, (2019). PMID: 30743181

6.Mcm8 and Mcm9 form a complex that functions in homologous recombination repair induced by DNA interstrand crosslinks. Nishimura K, Ishiai M, Horikawa K, Fukagawa T, Takata M, Takisawa H, Kanemaki MT. Mol. Cell 47, 511-22, (2012). PMID: 22771115

Integrated to
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.