PR01660

MCMPROTEIN4

PRINTS entry
Member databasePRINTS
PRINTS typefamily
Short nameMCMPROTEIN4

Description
Imported from IPR008047

The MCM2-7 complex consists of six closely related proteins that are highly conserved throughout the eukaryotic kingdom. In eukaryotes, Mcm3 is a component of the MCM2-7 complex (MCM complex), which consists of six sequence-related AAA + type ATPases/helicases that form a hetero-hexameric ring
[2]
. MCM2-7 complex is part of the pre-replication complex (pre-RC). In G1 phase, inactive MCM2-7 complex is loaded onto origins of DNA replication
[5, 3, 1]
. During G1-S phase, MCM2-7 complex is activated to unwind the double stranded DNA and plays an important role in DNA replication forks elongation
[1]
.

The components of the MCM2-7 complex are:


 * DNA replication licensing factor MCM2,
IPR008045

 * DNA replication licensing factor MCM3,
IPR008046

 * DNA replication licensing factor MCM4,
IPR008047

 * DNA replication licensing factor MCM5,
IPR008048

 * DNA replication licensing factor MCM6,
IPR008049

 * DNA replication licensing factor MCM7,
IPR008050



Mcm4 is thought to play a pivotal role in ensuring DNA replication occurs only once per cell cycle. Phosphorylation of Mcm4 dramatically reduces its affinity for chromatin - it has been proposed that this cell cycle-dependent phosphorylation is the mechanism that inactivates the MCM complex from late S phase through mitosis, thus preventing illegitimate DNA replication during that period of the cell cycle
[4]
.

References
Imported from IPR008047

1.Control over DNA replication in time and space. Symeonidou IE, Taraviras S, Lygerou Z. FEBS Lett. 586, 2803-12, (2012). View articlePMID: 22841721

2.Structure and mechanism of helicases and nucleic acid translocases. Singleton MR, Dillingham MS, Wigley DB. Annu. Rev. Biochem. 76, 23-50, (2007). View articlePMID: 17506634

3.A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Evrin C, Clarke P, Zech J, Lurz R, Sun J, Uhle S, Li H, Stillman B, Speck C. Proc. Natl. Acad. Sci. U.S.A. 106, 20240-5, (2009). View articlePMID: 19910535

4.Phosphorylation of MCM4 by cdc2 protein kinase inhibits the activity of the minichromosome maintenance complex. Hendrickson M, Madine M, Dalton S, Gautier J. Proc. Natl. Acad. Sci. U.S.A. 93, 12223-8, (1996). View articlePMID: 8901561

5.Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF. Cell 139, 719-30, (2009). View articlePMID: 19896182

Supplementary References

1. Initiation of chromosomal DNA replication in eukaryotes. Lessons from lambda. Stillman B. J. Biol. Chem. 269, 7047-50, (1994). View articlePMID: 8125908

2. S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative state. Dahmann C, Diffley JF, Nasmyth KA. Curr. Biol. 5, 1257-69, (1995). View articlePMID: 8574583

3. Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. Kubota Y, Mimura S, Nishimoto S, Masuda T, Nojima H, Takisawa H. EMBO J. 16, 3320-31, (1997). View articlePMID: 9214647

4. Zinc fingers are sticking together. Mackay JP, Crossley M. Trends Biochem. Sci. 23, 1-4, (1998). View articlePMID: 9478126

5. Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells. Diffley JF. Genes Dev. 10, 2819-30, (1996). View articlePMID: 8918884

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