PS50003

PH domain profile

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Short namePH_DOMAIN

Description

The 'pleckstrin homology' (PH) domain is a domain of about 100 residues that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton
[8]
[7]
[3]
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. The function of this domain is not clear, several putative functions have been suggested: - binding to the beta/gamma subunit of heterotrimeric G proteins, - binding to lipids, e.g. phosphatidylinositol-4,5-bisphosphate, - binding to phosphorylated Ser/Thr residues, - attachment to membranes by an unknown mechanism. It is possible that different PH domains have totally different ligand requirements. The 3D structure of several PH domains has been determined
[2]
. All known cases have a common structure consisting of two perpendicular anti-parallel beta sheets, followed by a C-terminal amphipathic helix. The loops connecting the beta-strands differ greatly in length, making the PH domain relatively difficult to detect. There are no totally invariant residues within the PH domain. Proteins reported to contain one more PH domains belong to the following families: - Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. Pleckstrin is one of the rare proteins to contains two PH domains. - Ser/Thr protein kinases such as the Act/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. - Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. - Insulin Receptor Substrate 1 (IRS-1). - Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and yeast CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. - Cytoskeletal proteins such as dynamin, Caenorhabditis elegans kinesin-like protein unc-104, spectrin beta- chain, syntrophin (2 PH domains) and yeast nuclear migration protein NUM1. - Mammalian phosphatidylinositol-specific phospholipase C (PI-PLC) isoforms gamma and delta. Isoform gamma contains two PH domains, the second one is split into two parts separated by about 400 residues. - Oxysterol binding proteins (OSBPs). - Mouse protein citron, a putative rho/rac effector that binds to the GTP- bound forms of rho and rac, - Several yeast proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). - Caenorhabditis elegans protein mig-10. - Caenorhabditis elegans hypothetical proteins C04D8.1, K06H7.4 and ZK632.12. - Yeast hypothetical proteins YBR129c and YHR155w. The profile for the PH domain, which has been developed by Toby Gibson at the EMBL, covers the total length of domain. Several proteins contain large insertions in the PH domain and are thus difficult to detect with this profile. In some of these cases, the profile will align only to one half of the PH domain.

References

1.Pleckstrin homology (PH) domains in signal transduction. Ingley E, Hemmings BA. J. Cell. Biochem. 56, 436-43, (1994). View articlePMID: 7890802

2.More meanders and sandwiches. Riddihough G. Nat. Struct. Biol. 1, 755-7, (1994). View articlePMID: 7634082

3.The PH domain: a common piece in the structural patchwork of signalling proteins. Musacchio A, Gibson T, Rice P, Thompson J, Saraste M. Trends Biochem. Sci. 18, 343-8, (1993). View articlePMID: 8236453

4.Pleckstrin homology domains: a fact file. Saraste M, Hyvonen M. Curr. Opin. Struct. Biol. 5, 403-8, (1995). View articlePMID: 7583640

5.PH domain: the first anniversary. Gibson TJ, Hyvonen M, Musacchio A, Saraste M, Birney E. Trends Biochem. Sci. 19, 349-53, (1994). View articlePMID: 7985225

6.Protein modules and signalling networks. Pawson T. Nature 373, 573-80, (1995). View articlePMID: 7531822

7.Pleckstrin domain homology. Haslam RJ, Koide HB, Hemmings BA. Nature 363, 309-10, (1993). View articlePMID: 8497315

8.A putative modular domain present in diverse signaling proteins. Mayer BJ, Ren R, Clark KL, Baltimore D. Cell 73, 629-30, (1993). View articlePMID: 8500161

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