PS50162

RecA family profile 1

PROSITE profiles entry
Member databasePROSITE profiles
PROSITE profiles typedomain
Short nameRECA_2

Description

The bacterial recA protein
[2]
[4]
is essential for homologous recombination and recombinational repair of DNA damage. RecA has many activities: it filaments, it binds to single- and double-stranded DNA, it binds and hydrolyzes ATP, it is also a recombinase and, finally, it interacts with lexA causing its activation and leading to its autocatalytic cleavage. RecA is a protein of about 350 amino-acid residues. Its sequence is very well conserved
[1]
[5]
among eubacterial species. It is also found in the chloroplast of plants
[3]
. The recA protein is closely related to: - Eukaryotic RAD51 protein. Promotes homologous pairing and strand exchange on chromatin. - Eukaryotic DMC1 protein. Participates in meiotic recombination. - Prokaryotic radA protein. Involved in DNA repair and in homologous recombination. - Bacteriophage uvsX gene product. Important in genetic recombination, DNA repair, and replication. As a signature pattern specific for the bacterial and chloroplastic recA protein, we selected the best conserved region, a nonapeptide located in the middle of the sequence and which is part of the monomer-monomer interface in a recA filament. We also developed two profiles. The first one covers the ATP binding domain in the N-terminal part of the recA protein. The second one span the whole monomer-monomer interface. These two profiles also pick up the recA-like proteins.

References

1.Bacterial classifications derived from recA protein sequence comparisons. Karlin S, Weinstock GM, Brendel V. J. Bacteriol. 177, 6881-93, (1995). View articlePMID: 7592482

2.recA-dependent DNA repair processes. Smith KC, Wang TC. Bioessays 10, 12-6, (1989). View articlePMID: 2653307

3.A homolog of Escherichia coli RecA protein in plastids of higher plants. Cerutti H, Osman M, Grandoni P, Jagendorf AT. Proc. Natl. Acad. Sci. U.S.A. 89, 8068-72, (1992). View articlePMID: 1518831

4.Evolution of the recA gene and the molecular phylogeny of bacteria. Lloyd AT, Sharp PM. J. Mol. Evol. 37, 399-407, (1993). View articlePMID: 8308907

5.The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species. Eisen JA. J. Mol. Evol. 41, 1105-23, (1995). View articlePMID: 8587109

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