Member database | PROSITE profiles |
PROSITE profiles type | domain |
Short name | NFYA_HAP2_2 |
Description
Diverse DNA binding proteins are known to bind the CCAAT box, a common cis-
acting element found in the promoter and enhancer regions of a large number of
genes in eukaryotes. Amongst these proteins is one known as the CCAAT-binding
factor (CBF) or NF-Y
[2]. CBF is a heteromeric transcription factor that
consists of two different components both needed for DNA-binding.
The HAP protein complex of yeast binds to the upstream activation site of
cytochrome C iso-1 gene (CYC1) as well as other genes involved in
mitochondrial electron transport and activates their expression. It also
recognizes the sequence CCAAT and is structurally and evolutionary related to
CBF.
The first subunit of CBF, known as CBF-A or NF-YB in vertebrates and HAP3 in
budding yeast, is a protein of 116 to 210 amino- acid residues which contains
a highly conserved central domain of about 90 residues. This domain displays
some sequence similarity to histones H2B and seems to be
involved in DNA-binding; we have developed a signature pattern from its
central part.
The second subunit of CBF, known as CBF-B or NF-YA in vertebrates and HAP2 in
budding yeast, is a protein of 265 to 350 amino-acid residues which contains a
highly conserved region of about 60 residues. This region, called the
'essential core'
[1], seems to consist of two subdomains: an N-terminal
subunit-association domain and a C-terminal DNA recognition domain. We have
developed a signature pattern from a section of the subunit-association
domain (SAD) and a profile that covers both subdomains.
References
1.The Schizosaccharomyces pombe homolog of Saccharomyces cerevisiae HAP2 reveals selective and stringent conservation of the small essential core protein domain. Olesen JT, Fikes JD, Guarente L. Mol. Cell. Biol. 11, 611-9, (1991). View articlePMID: 1899284
2.Evolutionary variation of the CCAAT-binding transcription factor NF-Y. Li XY, Mantovani R, Hooft van Huijsduijnen R, Andre I, Benoist C, Mathis D. Nucleic Acids Res. 20, 1087-91, (1992). View articlePMID: 1549471