Member database | PROSITE patterns |
PROSITE patterns type | conserved site |
Short name | COMPLEX1_24K |
Description
Respiratory-chain NADH dehydrogenase (EC 1.6.5.3)
[2] (also known as complex
I or NADH-ubiquinone oxidoreductase) is an oligomeric enzymatic complex
located in the inner mitochondrial membrane which also seems to exist in
the chloroplast and in cyanobacteria (as a NADH-plastoquinone oxidoreductase).
Among the 25 to 30 polypeptide subunits of this bioenergetic enzyme complex
there is one with a molecular weight of 24 Kd (in mammals), which is a
component of the iron-sulfur (IP) fragment of the enzyme. It seems to bind a
2Fe-2S iron-sulfur cluster. The 24 Kd subunit is nuclear encoded, as a
precursor form with a transit peptide in mammals, and in Neurospora crassa.
The 24 Kd subunit is highly similar to
[3][1]:
- Subunit E of Escherichia coli NADH-ubiquinone oxidoreductase (gene nuoE).
- Subunit NQO2 of Paracoccus denitrificans NADH-ubiquinone oxidoreductase.
As as signature pattern we selected a highly conserved region, located in the
central section of this subunit and which contains two conserved cysteines
that are probably involved in the binding of the 2Fe-2S center.
References
1.The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I. Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H. J. Mol. Biol. 233, 109-22, (1993). View articlePMID: 7690854
2.The respiratory-chain NADH dehydrogenase (complex I) of mitochondria. Weiss H, Friedrich T, Hofhaus G, Preis D. Eur. J. Biochem. 197, 563-76, (1991). View articlePMID: 2029890
3.Conservation of sequences of subunits of mitochondrial complex I and their relationships with other proteins. Fearnley IM, Walker JE. Biochim. Biophys. Acta 1140, 105-34, (1992). View articlePMID: 1445936