PS01150

Respiratory-chain NADH dehydrogenase 20 Kd subunit signature

PROSITE patterns entry
Member databasePROSITE patterns
PROSITE patterns typeconserved site
Short nameCOMPLEX1_20K

Description

Respiratory-chain NADH dehydrogenase (EC 1.6.5.3)
[3]
(also known as complex I or NADH-ubiquinone oxidoreductase) is an oligomeric enzymatic complex located in the inner mitochondrial membrane which also seems to exist in the chloroplast and in cyanobacteria (as a NADH-plastoquinone oxidoreductase). Among the 25 to 30 polypeptide subunits of this bioenergetic enzyme complex there is one with a molecular weight of 20 Kd (in mammals)
[2]
, which is a component of the iron-sulfur (IP) fragment of the enzyme. It seems to bind a 4Fe-4S iron-sulfur cluster. The 20 Kd subunit has been found to be: - Nuclear encoded, as a precursor form with a transit peptide in mammals, and in Neurospora crassa. - Mitochondrial encoded in Paramecium (gene psbG). - Chloroplast encoded in various higher plants (gene ndhK or psbG). The 20 Kd subunit is highly similar to
[1]
: - Synechocystis strain PCC 6803 proteins psbG1 and psbG2. - Subunit B of Escherichia coli NADH-ubiquinone oxidoreductase (gene nuoB). - Subunit NQO6 of Paracoccus denitrificans NADH-ubiquinone oxidoreductase. - Subunit 7 of Escherichia coli formate hydrogenlyase (gene hycG). - Subunit I of Escherichia coli hydrogenase-4 (gene hyfI). As as signature pattern we selected a highly conserved region, located in the central section of this subunit and which contains a conserved cysteine that is probably involved in the binding of the 4Fe-4S center.

References

1.The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I. Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H. J. Mol. Biol. 233, 109-22, (1993). View articlePMID: 7690854

2.NADH: ubiquinone oxidoreductase from bovine heart mitochondria. A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes. Arizmendi JM, Runswick MJ, Skehel JM, Walker JE. FEBS Lett. 301, 237-42, (1992). View articlePMID: 1577158

3.The respiratory-chain NADH dehydrogenase (complex I) of mitochondria. Weiss H, Friedrich T, Hofhaus G, Preis D. Eur. J. Biochem. 197, 563-76, (1991). View articlePMID: 2029890

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