Family C48
Summary for family C48
Name | Peptidase family C48 (Ulp1endopeptidase family) |
---|---|
Family type peptidase | C48.001 - Ulp1 peptidase (Saccharomyces cerevisiae), MEROPS Accession MER0005202 (peptidase unit: 439-592) |
Content of family | Peptidase family C48 contains SUMO (small ubiquitin-like modifier) deconjugating enzymes. |
History |
Identifier created: MEROPS 5.00 (20 April 2000) SUMO proteins are a group of proteins that are related to ubiquitin both in their structure and way in which they are ligated. However, unlike ubiquitinated proteins, sumolated proteins do not tend to be degraded. Instead SUMO and Smt3 (the yeast equivalent) have been implicated in the targeting of protein for export from the nucleus. The members of peptidase family C48 are involved in the processing of SUMO precursors and in the deconjugation of SUMO proteins from cellular targets. Families C97 and C98 also contain peptidases that release SUMO from conjugated proteins (Hickey et al., 2012). |
Catalytic type | Cysteine |
Active site residues | H514 D531 Q574 C580 |
Active site | The catalytic Cys580 is coordinated by His514, which is stabilized by Asp531. Cys580 and Gln574 form the oxyanion hole (Mossenova & Lima, 2000). |
Activities and specificities | Ulp1 peptidase (C48.001) demonstrates dual enzymic activity different to most deubiquitinating enzymes. Firstly, Ulp1 processes the Smt3 precursor into its mature form, cleaving after a Gly-Gly motif. Secondly, Ulp1 cleaves the peptide bond formed between the C-terminal Gly of the mature Smt3 and the lysine epsilon-amino groups of target proteins. It has been demonstrated that Ulp1 can recognise the substrate of SENP1 (C48.002), SUMO-1 (Li & Hochstrasser, 1999). |
Inhibitors | Ulp1 peptidase is sensitive to inhibition by ubiquitin aldehyde (Lima, 2004). |
Molecular structure | Ulp1 peptidase shows similarity to the tertiary structure of adenain (C05.001). Trp448 and Trp515 enable the formation of a constricted VDW tunnel to interact with the C-terminal Smt3 Gly-Gly motif (Mossenova & Lima, 2000). Surprisingly, the three families of deSUMOlatying peptidases are members of three different clans despite being the same catalytic type. |
Clan | CE |
Basis of clan assignment | Protein fold of the peptidase unit for members of this family resembles that of adenain, the type example for clan CE. |
Distribution of family | Bacteria | details | |
Archaea | details | ||
Protozoa | details | ||
Fungi | - | ||
Plants | details | ||
Animals | details | ||
Viruses | details |
Biological functions | Suggested roles for SUMO type proteins include the regulation of nucleocytoplasmic transport or in processes coordinating SUMO modification on entry or exit from the nucleus via the nuclear pore complex protein RANB2 (Lima, 2004). | |
Statistics for family C48 | Sequences: | 5778 |
Identifiers: | 59 | |
Identifiers with PDB entries: | 10 | |
Downloadable files | Sequence library (FastA format) | |
Sequence alignment (FastA format) | ||
Phylogenetic tree (Newick format) |
Other databases | CATH | 3.10.20.90 | ||
INTERPRO | IPR003653 | |||
PANTHER | PTHR12606 | |||
PANTHER | PTHR44655 | |||
PANTHER | PTHR44784 | |||
PANTHER | PTHR45370 | |||
PFAM | PF02902 | |||
SCOP | 54054 |
Peptidases and Homologues | MEROPS ID | Structure |
---|---|---|
Ulp1 peptidase | C48.001 | Yes |
SENP1 peptidase | C48.002 | Yes |
SENP3 peptidase | C48.003 | - |
SENP6 peptidase | C48.004 | - |
Ulp2 peptidase | C48.005 | Yes |
SENP2 peptidase | C48.007 | Yes |
SENP5 peptidase | C48.008 | - |
SENP7 peptidase | C48.009 | Yes |
SUSP4 peptidase (Mus musculus) | C48.010 | - |
SENP8 peptidase | C48.011 | Yes |
SENP4 peptidase | C48.012 | - |
Mername-AA145 peptidase | C48.013 | - |
Mername-AA147 peptidase | C48.014 | - |
Mername-AA148 peptidase | C48.015 | - |
Mername-AA149 peptidase | C48.016 | - |
Mername-AA150 peptidase | C48.017 | - |
peptidase similar to SUMO-1-specific peptidase (Rattus norvegicus) | C48.018 | - |
LOC297623 peptidase (Rattus norvegicus) | C48.020 | - |
similar to SUMO-1-specific peptidase (Mus musculus) | C48.021 | - |
ESD4 peptidase | C48.022 | - |
XopD peptidase | C48.023 | Yes |
Ulp1 peptidase (Drosophila-type) | C48.024 | - |
Nep1 peptidase (Schizosaccharomyces sp.) | C48.025 | - |
573K1.5 peptidase | C48.026 | - |
Mername-AA233 putative peptidase (Mus musculus) | C48.027 | - |
sentrin-specific peptidase 14 (Mus musculus) | C48.028 | - |
sentrin-specific peptidase 15 (Mus musculus) | C48.029 | - |
sentrin-specific peptidase 17 (Rattus norvegicus) | C48.030 | - |
SENP8 peptidase (fungus-type) | C48.031 | - |
ChlaDub1 peptidase | C48.032 | Yes |
ChlaDub2 peptidase | C48.033 | - |
PfSENP1 (Plasmodium falciparum) | C48.034 | - |
ASP1 peptidase (Arabidopsis thaliana) | C48.035 | - |
Mername-AA146 protein | C48.971 | - |
At4g00690 (Arabidopsis thaliana) | C48.A01 | - |
At3g06910 (Arabidopsis thaliana) | C48.A02 | - |
At5g60190 (Arabidopsis thaliana) | C48.A03 | - |
At1g60220 (Arabidopsis thaliana) | C48.A04 | - |
At1g10570 (Arabidopsis thaliana) | C48.A05 | - |
CG12717 g.p. (Drosophila melanogaster) | C48.A06 | - |
CG10107 g.p. (Drosophila melanogaster) | C48.A07 | - |
CG11023 g.p. (Drosophila melanogaster) | C48.A08 | - |
ulp2 protein (Schizosaccharomyces pombe-type) | C48.A09 | - |
ulp-7 g.p. (Caenorhabditis elegans) | C48.A10 | - |
T27C5.9 transposon (Caenorhabditis elegans) | C48.A11 | - |
ulp-2 g.p. (Caenorhabditis elegans) | C48.A12 | - |
ulp-3 g.p. (Caenorhabditis elegans) | C48.A13 | - |
Y48A5A.1 g.p. (Caenorhabditis elegans) | C48.A14 | - |
ulp-1 g.p. (Caenorhabditis elegans) | C48.A15 | - |
ulp-4 g.p. (Caenorhabditis elegans) | C48.A16 | - |
DDB_G0293508 (Dictyostelium discoideum) | C48.A17 | - |
DDB_G0292290 (Dictyostelium discoideum) | C48.A18 | - |
ulp1 peptidase (Schizosaccharomyces pombe-type) | C48.A19 | - |
MAL8P1.157 g.p. (Plasmodium falciparum) | C48.A20 | - |
LOC559077 g.p. (Brachydanio rerio) | C48.A21 | - |
LOC571373 g.p. (Brachydanio rerio) | C48.A22 | - |
SPF2 (Arabidopsis thaliana) | C48.A23 | - |
pseudogene (Mus musculus chromosome 2) | C48.P01 | - |
LOC625377 pseudogene (Mus musculus chromosome 14) | C48.P02 | - |
Family C48 non-peptidase homologues | non-peptidase homologue | Yes |
Family C48 unassigned peptidases | unassigned | Yes |